ArticleProceedings of the National Academy of Sciences of the United States of America2009
Ctp1 and Exonuclease 1, alternative nucleases regulated by the MRN complex, are required for efficient meiotic recombination.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 53 citations in OpenAlex.
- Divergence and conservation of the meiotic recombination machinery.Nature reviews. Genetics · 2024Review
- MRE11A: a novel negative regulator of human DNA mismatch repair.Cellular & molecular biology letters · 2024Article
- The MRN complex and topoisomerase IIIa-RMI1/2 synchronize DNA resection motor proteins.The Journal of biological chemistry · 2023Article
- Regulation Mechanisms of Meiotic Recombination Revealed from the Analysis of a Fission Yeast Recombination HotspotBiomolecules · 2022Review
- OsRAD51 Plays a Vital Role in Promoting Homologous Recombination in Rice Meiosis.International journal of molecular sciences · 2022Article
- Review
- DNA sequence differences are determinants of meiotic recombination outcome.Scientific reports · 2019Article
- XPG-related nucleases are hierarchically recruited for double-stranded rDNA break resection.The Journal of biological chemistry · 2019Article
- CDK contribution to DSB formation and recombination in fission yeast meiosis.PLoS genetics · 2019Article
- Human Exonuclease 1 (EXO1) Regulatory Functions in DNA Replication with Putative Roles in Cancer.International journal of molecular sciences · 2018Review
- A Heterochromatin Domain Forms Gradually at a New Telomere and Is Dynamic at Stable Telomeres.Molecular and cellular biology · 2018Article
- Eukaryotic resectosomes: A single-molecule perspective.Progress in biophysics and molecular biology · 2017Review
- Next-Generation DNA Curtains for Single-Molecule Studies of Homologous Recombination.Methods in enzymology · 2017Article
- OsDMC1 Is Not Required for Homologous Pairing in Rice Meiosis.Plant physiology · 2016Article
- Article
- Two separable functions of Ctp1 in the early steps of meiotic DNA double-strand break repair.Nucleic acids research · 2015Article
- Initiation of meiotic homologous recombination: flexibility, impact of histone modifications, and chromatin remodeling.Cold Spring Harbor perspectives in biology · 2015Review
- Casein Kinase 1 and Phosphorylation of Cohesin Subunit Rec11 (SA3) Promote Meiotic Recombination through Linear Element Formation.PLoS genetics · 2015Article
- Tetrameric Ctp1 coordinates DNA binding and DNA bridging in DNA double-strand-break repair.Nature structural & molecular biology · 2015Article
- Increased meiotic crossovers and reduced genome stability in absence of Schizosaccharomyces pombe Rad16 (XPF).Genetics · 2014Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Double-strand breaks (DSBs) in DNA are lethal unless repaired. Faithful repair requires processing of the DSB ends and interaction with intact homologous DNA, which can produce genetic recombinants. To determine the role of nucleases in DSB end-processing and joint molecule resolution, we studied recombination at the site of a single DSB, generated by induction of the I-SceI endonuclease, during meiosis of fission yeast lacking Rec12 (Spo11 homolog) and, hence, other DSBs. We find that in the presence of the MRN (Rad32-Rad50-Nbs1) complex efficient recombination requires Ctp1, the ortholog of the nuclease Sae2, but not the nuclease activity of MRN. In the absence of MRN, exonuclease 1 (Exo1) becomes the major nuclease required for efficient recombination. Our data indicate that MRN enables access of Ctp1 to the DSB but blocks access of Exo1. In our assay, the Rad16-Swi10 nuclease, required for nucleotide excision-repair, is required for efficient recombination, presumably to remove heterologous DNA at the end of the I-SceI cut site. Another nuclease, the Mus81-Eme1 Holliday junction resolvase, is required to generate crossovers accompanying gene conversion at the I-SceI cut site. Additional, previously published evidence indicates that these 5 nucleases play similar roles in wild-type fission yeast meiotic recombination and in the repair of spontaneous and damage-induced mitotic DSBs. We propose that in wild-type meiosis MRN, in conjunction with Ctp1, removes the covalently attached Rec12 protein from the DNA end, which is then resected by Ctp1 and other activities to produce the single-stranded DNA necessary for further steps of DSB repair.
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