ArticleJournal of experimental & clinical cancer research : CR2009
Alpha6beta4 integrin crosslinking induces EGFR clustering and promotes EGF-mediated Rho activation in breast cancer.
Article in Journal of experimental & clinical cancer research : CR, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Protein-protein interactions as therapeutic targets in breast cancer: integrated computational and experimental advances, mechanisms and clinical translation.Molecular biology reports · 2026Review
- Nebivolol Inhibits Hepatocellular Carcinoma via RHOQ and Enhances the Efficacy of Lenvatinib.International journal of biological sciences · 2026Article
- Research progress of the netrins and their receptors in cancer.Journal of cellular and molecular medicine · 2024Review
- TM4SF1 promotes esophageal squamous cell carcinoma metastasis by interacting with integrin α6.Cell death & disease · 2022Article
- CD151 drives cancer progression depending on integrin α3β1 through EGFR signaling in non-small cell lung cancer.Journal of experimental & clinical cancer research : CR · 2021Article
- UNC5C‑knockdown enhances the growth and metastasis of breast cancer cells by potentiating the integrin α6/β4 signaling pathway.International journal of oncology · 2020Article
- Host-Defense Peptides Caerin 1.1 and 1.9 Stimulate TNF-Alpha-Dependent Apoptotic Signals in Human Cervical Cancer HeLa Cells.Frontiers in cell and developmental biology · 2020Article
- Integrin αBiomaterials · 2018Article
- Resveratrol suppresses breast cancer cell invasion by inactivating a RhoA/YAP signaling axis.Experimental & molecular medicine · 2017Article
- Integrins in the Spotlight of Cancer.International journal of molecular sciences · 2016Review
- N-Glycosylation of integrin α5 acts as a switch for EGFR-mediated complex formation of integrin α5β1 to α6β4.Scientific reports · 2016Article
- Distinct effects of β1 integrin on cell proliferation and cellular signaling in MDA-MB-231 breast cancer cells.Scientific reports · 2016Article
- Tuning cell migration: contractility as an integrator of intracellular signals from multiple cues.F1000Research · 2016Review
- Integrin α6β4 Promotes Autocrine Epidermal Growth Factor Receptor (EGFR) Signaling to Stimulate Migration and Invasion toward Hepatocyte Growth Factor (HGF).The Journal of biological chemistry · 2015Article
- ARF1 regulates the Rho/MLC pathway to control EGF-dependent breast cancer cell invasion.Molecular biology of the cell · 2014Article
- Integrin α5β1 facilitates cancer cell invasion through enhanced contractile forces.Journal of cell science · 2011Article
- β1-integrins signaling and mammary tumor progression in transgenic mouse models: implications for human breast cancer.Breast cancer research : BCR · 2011Review
- Biomarkers for Basal-like Breast Cancer.Cancers · 2010Article
- Y-box binding protein-1 induces the expression of CD44 and CD49f leading to enhanced self-renewal, mammosphere growth, and drug resistance.Cancer research · 2010Article
- Alterations in integrin expression modulates invasion of pancreatic cancer cells.Journal of experimental & clinical cancer research : CR · 2009Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe alpha6beta4 integrin is overexpressed in the basal subtype of breast cancer and plays an important role in tumor cell motility and invasion. EGFR is also overexpressed in the basal subtype of breast cancer, and crosstalk between alpha6beta4 integrin and EGFR appears to be important in tumor progression.
methodsWe evaluated the effects of alpha6beta4 crosslinking on the distribution and function of EGFR in breast carcinoma cell line MDA-MB-231. Receptor distribution was evaluated by fluorescence microscopy and multispectral imaging flow cytometry, and ligand-mediated EGFR signaling was evaluated using Western blots and a Rho pull-down assay.
resultsAntibody-mediated crosslinking of alpha6beta4 integrin was sufficient to induce cell-surface clustering of not only alpha6beta4 but also EGFR in nonadherent cells. The induced clustering of EGFR was observed minimally after 5 min of integrin crosslinking but was more prominent after 15 min. EGFR clustering had minimal effect on the phosphorylation of Akt or Erk1,2 in response to EGF in suspended cells or in response to HB-EGF in adherent cells. However, EGFR clustering induced by crosslinking alpha6beta4 had a marked effect on Rho activation in response to EGF.
conclusionCrosslinking alpha6beta4 integrin in breast carcinoma cells induces EGFR clustering and preferentially promotes Rho activation in response to EGF. We hypothesize that this integrin-EGFR crosstalk may facilitate tumor cell cytoskeletal rearrangements important for tumor progression.
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