ArticleBMC genomics2009
Evaluation of methods for amplification of picogram amounts of total RNA for whole genome expression profiling.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.
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Who cites it
34 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Adapting INTACT to analyse cell-type-specific transcriptomes and nucleocytoplasmic mRNA dynamics in the Arabidopsis embryo.Plant reproduction · 2019Article
- Optimizing Laser Capture Microdissection Protocol for Isolating Zone-Specific Cell Populations from Mandibular Condylar Cartilage.International journal of dentistry · 2019Article
- Feasibility of developing reliable gene expression modules from FFPE derived RNA profiled on Affymetrix arrays.PloS one · 2018Article
- Robust transcriptional signatures for low-input RNA samples based on relative expression orderings.BMC genomics · 2017Article
- Transcriptome dynamics revealed by a gene expression atlas of the early Arabidopsis embryo.Nature plants · 2017Article
- Molecular Profile of Tumor-Specific CD8+ T Cell Hypofunction in a Transplantable Murine Cancer Model.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Evaluating whole transcriptome amplification for gene profiling experiments using RNA-Seq.BMC biotechnology · 2015Article
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- Transcriptome analyses of adult mouse brain reveal enrichment of lncRNAs in specific brain regions and neuronal populations.Frontiers in cellular neuroscience · 2015Article
- Laser capture microdissection in Ectocarpus siliculosus: the pathway to cell-specific transcriptomics in brown algae.Frontiers in plant science · 2015Article
- Mitochondrial oxidative phosphorylation transcriptome alterations in human amyotrophic lateral sclerosis spinal cord and blood.Neuromolecular medicine · 2014Article
- Transcriptome profiling of human hippocampus dentate gyrus granule cells in mental illness.Translational psychiatry · 2014Article
- Validation and implementation of a method for microarray gene expression profiling of minor B-cell subpopulations in man.BMC immunology · 2014Article
- Gene expression differences predict treatment outcome of merkel cell carcinoma patients.Journal of skin cancer · 2014Article
- Gene expression analyses of mouse aortic endothelium in response to atherogenic stimuli.Arteriosclerosis, thrombosis, and vascular biology · 2013Article
- Myc is required for β-catenin-mediated mammary stem cell amplification and tumorigenesis.Molecular cancer · 2013Article
Corrections and comments
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6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFor more than a decade, microarrays have been a powerful and widely used tool to explore the transcriptome of biological systems. However, the amount of biological material from cell sorting or laser capture microdissection is much too small to perform microarray studies. To address this issue, RNA amplification methods have been developed to generate sufficient targets from picogram amounts of total RNA to perform microarray hybridisation.
resultsIn this study, four commercial protocols for amplification of picograms amounts of input RNA for microarray expression profiling were evaluated and compared. The quantitative and qualitative performances of the methods were assessed. Microarrays were hybridised with the amplified targets and the amplification protocols were compared with respect to the quality of expression profiles, reproducibility within a concentration range of input RNA, and sensitivity. The results demonstrate significant differences between these four methods.
conclusionIn our hands, the WT-Ovation pico system proposed by Nugen appears to be the most suitable for RNA amplification. This comparative study will be useful to scientists needing to choose an amplification method to carry out microarray experiments involving samples comprising only a few cells and generating picogram amounts of RNA.
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