ArticleJournal of proteome research2009
Comprehensive analysis of phosphorylated proteins of Escherichia coli ribosomes.
Article in Journal of proteome research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 48 citations in OpenAlex.
- Phosphoproteomics reveals essential regulatory roles of phosphorylation in marine oligotrophic bacteria.Marine life science & technology · 2026Article
- On the Nature of the Last Common Ancestor: A Story from its Translation Machinery.Journal of molecular evolution · 2024Review
- Dynamic Protein Phosphorylation inMicroorganisms · 2024Article
- Molecular characterization and expression analysis ofSaudi journal of biological sciences · 2021Article
- Mirror-Image 5S Ribonucleoprotein Complexes.Angewandte Chemie (International ed. in English) · 2020Article
- Article
- Genome Wide Phosphoproteome Analysis ofFrontiers in microbiology · 2019Article
- Hornerin contains a Linked Series of Ribosome-Targeting Peptide Antibiotics.Scientific reports · 2018Article
- The role of bacterial protein tyrosine phosphatases in the regulation of the biosynthesis of secreted polysaccharides.Antioxidants & redox signaling · 2014Review
- Identification and characterization of CHCHD1, AURKAIP1, and CRIF1 as new members of the mammalian mitochondrial ribosome.Frontiers in physiology · 2013Article
- The Escherichia coli phosphotyrosine proteome relates to core pathways and virulence.PLoS pathogens · 2013Article
- Bacterial tyrosine kinases: evolution, biological function and structural insights.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- High-throughput identification and screening of novel Methylobacterium species using whole-cell MALDI-TOF/MS analysis.PloS one · 2012Article
- Strong cation exchange chromatography in analysis of posttranslational modifications: innovations and perspectives.Journal of biomedicine & biotechnology · 2011Review
- Cracking pre-40S ribosomal subunit structure by systematic analyses of RNA-protein cross-linking.The EMBO journal · 2010Article
- A compendium of human mitochondrial gene expression machinery with links to disease.Environmental and molecular mutagenesis · 2010Review
- NAD+-dependent deacetylase SIRT3 regulates mitochondrial protein synthesis by deacetylation of the ribosomal protein MRPL10.The Journal of biological chemistry · 2010Article
- Phosphorylated proteins of the mammalian mitochondrial ribosome: implications in protein synthesis.Journal of proteome research · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Phosphorylation of bacterial ribosomal proteins has been known for decades; however, there is still very limited information available on specific locations of the phosphorylation sites in ribosomal proteins and the role they might play in protein synthesis. In this study, we have mapped the specific phosphorylation sites in 24 Escherichia coli ribosomal proteins by tandem mass spectrometry. Detection of phosphorylation was achieved by either phosphorylation specific visualization techniques, ProQ staining, and antibodies for phospho-Ser, Thr, and Tyr; or by mass spectrometry equipped with a capability to detect addition and loss of the phosphate moiety. Enrichment by immobilized metal affinity and/or strong cation exchange chromatography was used to improve the success of detection of the low abundance phosphopeptides. We found the small subunit (30S) proteins S3, S4, S5, S7, S11, S12, S13, S18, and S21 and the large subunit (50S) proteins L1, L2, L3, L5, L6, L7/L12, L13, L14, L16, L18, L19, L21, L22, L28, and L31 to be phosphorylated at one or more residues. Potential roles for each specific site in ribosome function were deduced through careful evaluation of the given phosphorylation sites in 3D-crystal structure models of ribosomes and the previous mutational studies of E. coli ribosomal proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.