ArticleBMC genomics2009
A White Campion (Silene latifolia) floral expressed sequence tag (EST) library: annotation, EST-SSR characterization, transferability, and utility for comparative mapping.
Article in BMC genomics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 56 citations in OpenAlex.
- Characterization of leaf transcriptome, development and utilization of unigenes-derived microsatellite markers in sugarcane (Physiology and molecular biology of plants : an international journal of functional plant biology · 2018Article
- Investigating past range dynamics for a weed of cultivation, Silene vulgaris.Ecology and evolution · 2016Article
- Evidence for asymmetrical hybridization despite pre- and post-pollination reproductive barriers between two Silene species.AoB PLANTS · 2016Article
- Population Genetics of the Rubber-Producing Russian Dandelion (Taraxacum kok-saghyz).PloS one · 2016Article
- Size Does Matter: Staging of Silene latifolia Floral Buds for Transcriptome Studies.International journal of molecular sciences · 2015Article
- Sex and parasites: genomic and transcriptomic analysis of Microbotryum lychnidis-dioicae, the biotrophic and plant-castrating anther smut fungus.BMC genomics · 2015Article
- The structural and functional connectivity of the grassland plant Lychnis flos-cuculi.Heredity · 2014Article
- Patterns of cyto-nuclear linkage disequilibrium in Silene latifolia: genomic heterogeneity and temporal stability.Heredity · 2014Article
- Unravelling genetics at the top: mountain islands or isolated belts?Annals of botany · 2012Article
- Identification of white campion (Silene latifolia) guaiacol O-methyltransferase involved in the biosynthesis of veratrole, a key volatile for pollinator attraction.BMC plant biology · 2012Article
- Comparative mapping in the Fagaceae and beyond with EST-SSRs.BMC plant biology · 2012Article
- Analysis of expressed sequence tags from grapevine flower and fruit and development of simple sequence repeat markers.Molecular biology reports · 2012Article
- Comparative high-throughput transcriptome sequencing and development of SiESTa, the Silene EST annotation database.BMC genomics · 2011Article
- Mining functional microsatellites in legume unigenes.Bioinformation · 2011Article
- Analysis of expressed sequence tags from Prunus mume flower and fruit and development of simple sequence repeat markers.BMC genetics · 2010Article
- Complete switchgrass genetic maps reveal subgenome collinearity, preferential pairing and multilocus interactions.Genetics · 2010Article
- A full-length enriched cDNA library and expressed sequence tag analysis of the parasitic weed, Striga hermonthica.BMC plant biology · 2010Article
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundExpressed sequence tag (EST) databases represent a valuable resource for the identification of genes in organisms with uncharacterized genomes and for development of molecular markers. One class of markers derived from EST sequences are simple sequence repeat (SSR) markers, also known as EST-SSRs. These are useful in plant genetic and evolutionary studies because they are located in transcribed genes and a putative function can often be inferred from homology searches. Another important feature of EST-SSR markers is their expected high level of transferability to related species that makes them very promising for comparative mapping. In the present study we constructed a normalized EST library from floral tissue of Silene latifolia with the aim to identify expressed genes and to develop polymorphic molecular markers.
resultsWe obtained a total of 3662 high quality sequences from a normalized Silene cDNA library. These represent 3105 unigenes, with 73% of unigenes matching genes in other species. We found 255 sequences containing one or more SSR motifs. More than 60% of these SSRs were trinucleotides. A total of 30 microsatellite loci were identified from 106 ESTs having sufficient flanking sequences for primer design. The inheritance of these loci was tested via segregation analyses and their usefulness for linkage mapping was assessed in an interspecific cross. Tests for crossamplification of the EST-SSR loci in other Silene species established their applicability to related species.
conclusionThe newly characterized genes and gene-derived markers from our Silene EST library represent a valuable genetic resource for future studies on Silene latifolia and related species. The polymorphism and transferability of EST-SSR markers facilitate comparative linkage mapping and analyses of genetic diversity in the genus Silene.
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