ArticlePlant molecular biology2009
Targeted mutagenesis in the progeny of maize transgenic plants.
Article in Plant molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 37 citations in OpenAlex.
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
- Challenges and Perspectives in Homology-Directed Gene Targeting in Monocot Plants.Rice (New York, N.Y.) · 2019Review
- Cas9-Guide RNA Directed Genome Editing in Soybean.Plant physiology · 2015Article
- Genome editing for crop improvement: Challenges and opportunities.GM crops & food · 2015Article
- Precision genome engineering and agriculture: opportunities and regulatory challenges.PLoS biology · 2014Article
- Advanced genetic tools for plant biotechnology.Nature reviews. Genetics · 2013Review
- Targeted DNA excision in Arabidopsis by a re-engineered homing endonuclease.BMC biotechnology · 2012Article
- Article
- Creating highly specific nucleases by fusion of active restriction endonucleases and catalytically inactive homing endonucleases.Nucleic acids research · 2012Article
- Meganucleases and other tools for targeted genome engineering: perspectives and challenges for gene therapy.Current gene therapy · 2011Review
- Zinc-finger nucleases: a powerful tool for genetic engineering of animals.Transgenic research · 2010Review
- OptimizedIn vitro cellular & developmental biology. Plant : journal of the Tissue Culture AssociationArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have demonstrated that targeted mutagenesis can be accomplished in maize plants by excision, activation, and subsequent elimination of an endonuclease in the progeny of genetic crosses. The yeast FLP/FRT site-specific recombination system was used to excise and transiently activate the previously integrated yeast I-SceI homing endonuclease in maize zygotes and/or developing embryos. An artificial I-SceI recognition sequence integrated into genomic DNA was analyzed for mutations to indicate the I-SceI endonuclease activity. Targeted mutagenesis of the I-SceI site occurred in about 1% of analyzed F1 plants. Short deletions centered on the I-SceI-produced double-strand break were the predominant genetic lesions observed in the F1 plants. The I-SceI expression cassette was not detected in the mutant F1 plants and their progeny. However, the original mutations were faithfully transmitted to the next generation indicating that the mutations occurred early during the F1 plant development. The procedure offers simultaneous production of double-strand breaks and delivery of DNA template combined with a large number of progeny plants for future gene targeting experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.