ArticleAmerican journal of physiology. Heart and circulatory physiology2009
Regulation of gap junctional charge selectivity in cells coexpressing connexin 40 and connexin 43.
Article in American journal of physiology. Heart and circulatory physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Calcium Role in Gap Junction Channel Gating: Direct Electrostatic or Calmodulin-Mediated?International journal of molecular sciences · 2024Review
- The intercalated disc: a unique organelle for electromechanical synchrony in cardiomyocytes.Physiological reviews · 2023Review
- Patch clamp studies on TRPV4-dependent hemichannel activation in lens epithelium.Frontiers in pharmacology · 2023Article
- Retinal ganglion cell topography and spatial resolution estimation in the Japanese tree frog Hyla japonica (Günther, 1859).Journal of anatomy · 2019Article
- Nitric oxide, PKC-ε, and connexin43 are crucial for ischemic preconditioning-induced chemical gap junction uncoupling.Oncotarget · 2016Article
- Determinants of Cx43 Channel Gating and Permeation: The Amino Terminus.Biophysical journal · 2016Article
- Mix and match: investigating heteromeric and heterotypic gap junction channels in model systems and native tissues.FEBS letters · 2014Review
- Interfering amino terminal peptides and functional implications for heteromeric gap junction formation.Frontiers in pharmacology · 2013Article
- Structural basis for the selective permeability of channels made of communicating junction proteins.Biochimica et biophysica acta · 2013Review
- Inducible coexpression of connexin37 or connexin40 with connexin43 selectively affects intercellular molecular transfer.The Journal of membrane biology · 2012Article
- Neurons and β-cells of the pancreas express connexin36, forming gap junction channels that exhibit strong cationic selectivity.The Journal of membrane biology · 2012Article
- Intercellular calcium waves in primary cultured rat mesenteric smooth muscle cells are mediated by connexin43.Cell communication & adhesion · 2012Article
- Phosphorylation of connexin 50 by protein kinase A enhances gap junction and hemichannel function.The Journal of biological chemistry · 2011Article
- The shunt problem: control of functional shunting in normal and tumour vasculature.Nature reviews. Cancer · 2010Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Expression of connexin 40 (Cx40) and Cx43 in cardiovascular tissues varies as a function of age, injury, and development with unknown consequences on the selectivity of junctional communication and its acute regulation. We investigated the PKC-dependent regulation of charge selectivity in junctions composed of Cx43, Cx40, or both by simultaneous assessment of junctional permeance rate constants (B(dye)) for dyes of similar size but opposite charge, N,N,N-trimethyl-2-[methyl-(7-nitro-2,1,3-benzoxadiol-4-yl)amino]ethanaminium (NBD-M-TMA; +1) and Alexa 350 (-1). The ratio of dye rate constants (B(NBD-M-TMA)/B(Alexa 350)) indicated that Cx40 junctions are cation selective (10.7 +/- 0.5), whereas Cx43 junction are nonselective (1.22 +/- 0.14). In coexpressing cells, a broad range of junctional selectivities was observed with mean cation selectivity increasing as the Cx40 to Cx43 expression ratio increased. PKC activation reduced or eliminated dye permeability of Cx43 junctions without altering their charge selectivity, had no effect on either permeability or charge selectivity of Cx40 junctions, and significantly increased the cation selectivity of junctions formed by coexpressing cells (approaching charge selectivity of Cx40 junctions). Junctions composed of Cx43 truncated at residue 257 (Cx43tr) were also not charge selective, but when Cx43tr was coexpressed with Cx40, a broad range of junctional selectivities that was unaffected by PKC activation was observed. Thus, whereas the charge selectivities of homomeric/homotypic Cx43 and Cx40 junctions appear invariant, the selectivities of junctions formed by cells coexpressing Cx40 and Cx43 vary considerably, reflecting both their relative expression levels and phosphorylation-dependent regulation. Such regulation could represent a mechanism by which coexpressing cells such as vascular endothelium and atrial cells regulate acutely the selective intercellular communication mediated by their gap junctions.
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