ArticleAmerican journal of physiology. Lung cellular and molecular physiology2009
Immunotargeting and cloning of two CD34 variants exhibiting restricted expression in adult rat endothelia in vivo.
Article in American journal of physiology. Lung cellular and molecular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Function and mechanism of pyrin and IL-10 in the regulation of the inflammasome in pulmonary vascular endothelial cells following hemorrhagic shock.Experimental and therapeutic medicine · 2019Article
- The localization and distribution of cells labeled by a somatic stem cell-recognizing antibody (A3) in rat colon development; possible presence of a new cell type forming the intestinal stem cell niche.Journal of toxicologic pathology · 2019Article
- CD34 Promotes Pathological Epi-Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy.PloS one · 2016Article
- Genome-wide epigenetic and proteomic analysis reveals altered Notch signaling in EPC dysfunction.Physiological reports · 2015Article
- Preparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line.Advanced pharmaceutical bulletin · 2015Article
- In vivo proteomic imaging analysis of caveolae reveals pumping system to penetrate solid tumors.Nature medicine · 2014Article
- Is CD34 truly a negative marker for mesenchymal stromal cells?Cytotherapy · 2012Review
- Bone marrow mononuclear cells induce beneficial remodeling and reduce diastolic dysfunction in the left ventricle of hypertensive SS/MCWi rats.Physiological genomics · 2012Article
- Overcoming key technological challenges in using mass spectrometry for mapping cell surfaces in tissues.Molecular & cellular proteomics : MCP · 2011Review
- Construction of metabolically biotinylated adenovirus with deleted fiber knob as targeting vector.Virology journal · 2010Article
- Iodine-125 radiolabeling of silver nanoparticles for in vivo SPECT imaging.International journal of nanomedicine · 2010Article
- Lung vascular targeting using antibody to aminopeptidase P: CT-SPECT imaging, biodistribution and pharmacokinetic analysis.Journal of vascular research · 2010Article
- The new frontiers of the targeted interventions in the pulmonary vasculature: precision and safety (2017 Grover Conference Series).Pulmonary circulationArticle
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
Abstract
Mapping protein expression of endothelial cells (EC) in vivo is fundamental to understanding cellular function and may yield new tissue-selective targets. We have developed a monoclonal antibody, MAb J120, to a protein expressed primarily in rat lung and heart endothelium. The antigen was identified as CD34, a marker of hematopoietic stem cells and global marker of endothelial cells in human and mouse tissues. PCR-based cloning identified two CD34 variant proteins, full length and truncated, both of which are expressed on luminal endothelial cell plasma membranes (P) isolated from lung. Truncated CD34 predominated in heart P, and neither variant was detected in P from kidney or liver. CD34 in lung was readily accessible to (125)I-J120 inoculated intravenously, and immunohistochemistry showed strong CD34 expression in lung EC. Few microvessels stained in heart and kidney, and no CD34 was detected in vessels of other organs or in lymphatics. We present herein the first complete sequence of a rat CD34 variant and show for the first time that the encoded truncated variant is endogenously expressed on EC in vivo. We also demonstrate that CD34 expression in rat EC, unlike mouse and human, is restricted in its distribution enabling quite specific lung targeting in vivo.
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