ArticleToxicological sciences : an official journal of the Society of Toxicology2009
Characterization and interlaboratory comparison of a gene expression signature for differentiating genotoxic mechanisms.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed, 73 citations in OpenAlex.
- Review of Transcriptomic Biomarkers That Predict In Vitro Genotoxicity in Human Cell Lines.Environmental and molecular mutagenesis · 2025Review
- A Collaborative Initiative to Establish Genomic Biomarkers for Assessing Tumorigenic Potential to Reduce Reliance on Conventional Rodent Carcinogenicity Studies.Toxicological sciences : an official journal of the Society of Toxicology · 2022Article
- Discovery of novel antagonists targeting the DNA binding domain of androgen receptor by integrated docking-based virtual screening and bioassays.Acta pharmacologica Sinica · 2022Article
- Assessment of the performance of the TGx-DDI biomarker to detect DNA damage-inducing agents using quantitative RT-PCR in TK6 cells.Environmental and molecular mutagenesis · 2019Article
- TGx-DDI, a Transcriptomic Biomarker for Genotoxicity Hazard Assessment of Pharmaceuticals and Environmental Chemicals.Frontiers in big data · 2019Review
- Replication-dependent histone isoforms: a new source of complexity in chromatin structure and function.Nucleic acids research · 2018Review
- Gene and protein analysis reveals that p53 pathway is functionally inactivated in cytogenetically normal Acute Myeloid Leukemia and Acute Promyelocytic Leukemia.BMC medical genomics · 2017Article
- Tuning the metabolism of the anticancer drug cisplatin with chemoprotective agents to improve its safety and efficacy.Metallomics : integrated biometal science · 2016Review
- A Microchip for Integrated Single-Cell Gene Expression Profiling and Genotoxicity Detection.Sensors (Basel, Switzerland) · 2016Article
- Application of the TGx-28.65 transcriptomic biomarker to classify genotoxic and non-genotoxic chemicals in human TK6 cells in the presence of rat liver S9.Environmental and molecular mutagenesis · 2016Article
- Integration of metabolic activation with a predictive toxicogenomics signature to classify genotoxic versus nongenotoxic chemicals in human TK6 cells.Environmental and molecular mutagenesis · 2015Article
- Development of a toxicogenomics signature for genotoxicity using a dose-optimization and informatics strategy in human cells.Environmental and molecular mutagenesis · 2015Article
- Article
- Selectively targeting the DNA-binding domain of the androgen receptor as a prospective therapy for prostate cancer.The Journal of biological chemistry · 2014Article
- Cell-based biosensor to report DNA damage in micro- and nanosystems.Analytical chemistry · 2014Article
- A metabolomics investigation of non-genotoxic carcinogenicity in the rat.Journal of proteome research · 2013Article
- Identification of Stage-Specific Gene Expression Signatures in Response to Retinoic Acid during the Neural Differentiation of Mouse Embryonic Stem Cells.Frontiers in genetics · 2012Article
- Hepatic mRNA, microRNA, and miR-34a-target responses in mice after 28 days exposure to doses of benzo(a)pyrene that elicit DNA damage and mutation.Environmental and molecular mutagenesis · 2012Article
- Human embryonic stem cell derived hepatocyte-like cells as a tool for in vitro hazard assessment of chemical carcinogenicity.Toxicological sciences : an official journal of the Society of Toxicology · 2011Article
- Genetic toxicology in the 21st century: reflections and future directions.Environmental and molecular mutagenesis · 2011Review
Corrections and comments
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Authors and funding
18 authors at 11 institutions in 5 countries.
Funding
Abstract
The genotoxicity testing battery is highly sensitive for detection of chemical carcinogens. However, it features a low specificity and provides only limited mechanistic information required for risk assessment of positive findings. This is especially important in case of positive findings in the in vitro chromosome damage assays, because chromosome damage may be also induced secondarily to cell death. An increasing body of evidence indicates that toxicogenomic analysis of cellular stress responses provides an insight into mechanisms of action of genotoxicants. To evaluate the utility of such a toxicogenomic analysis we evaluated gene expression profiles of TK6 cells treated with four model genotoxic agents using a targeted high density real-time PCR approach in a multilaboratory project coordinated by the Health and Environmental Sciences Institute Committee on the Application of Genomics in Mechanism-based Risk Assessment. We show that this gene profiling technology produced reproducible data across laboratories allowing us to conclude that expression analysis of a relevant gene set is capable of distinguishing compounds that cause DNA adducts or double strand breaks from those that interfere with mitotic spindle function or that cause chromosome damage as a consequence of cytotoxicity. Furthermore, our data suggest that the gene expression profiles at early time points are most likely to provide information relevant to mechanisms of genotoxic damage and that larger gene expression arrays will likely provide richer information for differentiating molecular mechanisms of action of genotoxicants. Although more compounds need to be tested to identify a robust molecular signature, this study confirms the potential of toxicogenomic analysis for investigation of genotoxic mechanisms.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.