ArticleNucleic acids research2009
RFXB and its splice variant RFXBSV mediate the antagonism between IFNgamma and TGFbeta on COL1A2 transcription in vascular smooth muscle cells.
Article in Nucleic acids research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Exploring the Role of Obesity in Dilated Cardiomyopathy Based on Bio-informatics Analysis.Journal of cardiovascular development and disease · 2022Article
- Forkhead transcription factor FOXO3a mediates interferon-γ-induced MHC II transcription in macrophages.Immunology · 2019Article
- HADC5 deacetylates MKL1 to dampen TNF-α induced pro-inflammatory gene transcription in macrophages.Oncotarget · 2017Article
- The histone H3K9 methyltransferase SUV39H links SIRT1 repression to myocardial infarction.Nature communications · 2017Article
- Role of Epigenetic Histone Modifications in Diabetic Kidney Disease Involving Renal Fibrosis.Journal of diabetes research · 2017Review
- Myocardin-Related Transcription Factor A Epigenetically Regulates Renal Fibrosis in Diabetic Nephropathy.Journal of the American Society of Nephrology : JASN · 2015Article
- Class II transactivator (CIITA) mediates transcriptional repression of pdk4 gene by interacting with hypermethylated in cancer 1 (HIC1).Journal of biomedical research · 2015Article
- Megakaryocytic leukemia 1 (MKL1) regulates hypoxia induced pulmonary hypertension in rats.PloS one · 2014Article
- MRTF-A steers an epigenetic complex to activate endothelin-induced pro-inflammatory transcription in vascular smooth muscle cells.Nucleic acids research · 2014Article
- Megakaryocytic leukemia 1 (MKL1) ties the epigenetic machinery to hypoxia-induced transactivation of endothelin-1.Nucleic acids research · 2013Article
- Interferon gamma (IFN-γ) disrupts energy expenditure and metabolic homeostasis by suppressing SIRT1 transcription.Nucleic acids research · 2012Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokines secreted by infiltrating immune cells during atherogenesis modulate vascular remodeling. One exemplary event is the antagonism between transformed growth factor (TGF-beta) and interferon gamma (IFN-gamma) on the transcriptional control of type I collagen gene (COL1A2). Previously we have reported that IFN-gamma up-regulates regulatory factor for X-box B (RFXB) to repress collagen transcription while down-regulates the expression of RFXBSV, a splice variant of RFXB that blocks collagen repression in fibroblasts. Here we demonstrate that TGF-beta abrogated COL1A2 repression by IFN-gamma through altering the relative expression of RFXB and RFXBSV. Unlike RFXB, RFXBSV did not bind to the collagen promoter and competed with RFXB for the co-repressor histone deacetylase 2 (HDAC2), limiting HDAC2 recruitment to the collagen transcription start site as evidenced by chromatin immunoprecipitation assays. Over-expression of RFXB by lentiviral infection in HASMCs enhanced HDAC2 enlistment, promoted histone deacetylation surrounding the collagen site by IFN-gamma, and blocked the TGF-beta antagonism, a pattern reversed by RFXBSV infection. On the contrary, silencing of RFXB, but not both RFXB and RFXBSV, expression promoted the TGF-beta antagonism. Thus, we have identified a novel mechanism whereby TGF-beta antagonizes the IFN-gamma repression of collagen transcription in HASMCs and as such provided new insights into antiatherogenic strategies.
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