ArticleExperimental neurology2010
An in vitro model of adult mammalian nerve repair.
Article in Experimental neurology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Mechanical Forces Guide Axon Growth through the Nigrostriatal Pathway in an Organotypic Model.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Magnetically-actuated microposts stimulate axon growth.Biophysical journal · 2022Article
- Review
- In Vitro Models for the Development of Peripheral Nerve Conduits, Part I: Design of a Fibrin Gel-Based Non-Contact Test.Polymers · 2021Article
- Sensory axons inhibit motor axon regeneration in vitro.Experimental neurology · 2020Article
- Glial Cell Line-Derived Neurotrophic Factor and Chondroitinase Promote Axonal Regeneration in a Chronic Denervation Animal Model.Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics · 2019Article
- Advances in ex vivo models and lab-on-a-chip devices for neural tissue engineering.Biomaterials · 2019Review
- Article
- Tissue Models for Neurogenesis and Repair in 3D.Advanced functional materials · 2018Article
- Vascular endothelial growth factor enhances axonal outgrowth in organotypic spinal cord slices via vascular endothelial growth factor receptor 1 and 2.Tissue engineering and regenerative medicine · 2016Article
- Organotypic Spinal Cord Culture: a Proper Platform for the Functional Screening.Molecular neurobiology · 2016Review
- 3D Printed Anatomical Nerve Regeneration Pathways.Advanced functional materials · 2015Article
- Femoral nerve regeneration and its accuracy under different injury mechanisms.Neural regeneration research · 2015Article
- GDNF preconditioning can overcome Schwann cell phenotypic memory.Experimental neurology · 2015Article
- Growth factor choice is critical for successful functionalization of nanoparticles.Frontiers in neuroscience · 2015Article
- A two-compartment organotypic model of mammalian peripheral nerve repair.Journal of neuroscience methods · 2014Article
- Novel roles for osteopontin and clusterin in peripheral motor and sensory axon regeneration.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- A microchip for quantitative analysis of CNS axon growth under localized biomolecular treatments.Journal of neuroscience methods · 2014Article
- Biofabrication and testing of a fully cellular nerve graft.Biofabrication · 2013Article
- Generation of magnetized olfactory ensheathing cells for regenerative studies in the central and peripheral nervous tissue.International journal of molecular sciences · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
The role of pathway-derived growth factors in the support of peripheral axon regeneration remains elusive. Few appropriate knock-out mice are available, and gene silencing techniques are rarely 100% effective. To overcome these difficulties, we have developed an in vitro organotypic co-culture system that accurately models peripheral nerve repair in the adult mammal. Spinal cord sections from P4 mice that express YFP in their neurons are used to innervate segments of P4 peripheral nerve. This reconstructed ventral root is then transected and joined to a nerve graft. Growth of axons across the nerve repair and into the graft can be imaged repeatedly with fluorescence microscopy to define regeneration speed, and parent neurons can be labeled in retrograde fashion to identify contributing neurons. Nerve graft harvested from adult mice remains viable in culture by both morphologic and functional criteria. Motoneurons are supported with GDNF for the first week in culture, after which they survive axotomy, and are thus functionally adult. This platform can be modified by using motoneurons from any genetically modified mouse that can be bred to express XFP, by harvesting nerve graft from any source, or by treating the culture systemically with antibodies, growth factors, or pathway inhibitors. The regeneration environment is controlled to a degree not possible in vivo, and the use of experimental animals is reduced substantially. The flexibility and control offered by this technique should thus make it a useful tool for the study of regeneration biology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.