ArticleProceedings of the National Academy of Sciences of the United States of America1991
GCR1 of Saccharomyces cerevisiae encodes a DNA binding protein whose binding is abolished by mutations in the CTTCC sequence motif.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 126 citations in OpenAlex.
- Rewiring regulation on respiro-fermentative metabolism relieved Crabtree effects inSynthetic and systems biotechnology · 2022Article
- Differential activation mechanisms of two isoforms of Gcr1 transcription factor generated from spliced and un-spliced transcripts in Saccharomyces cerevisiae.Nucleic acids research · 2021Article
- Quantitative analysis of transcription factor binding and expression using calling cards reporter arrays.Nucleic acids research · 2020Article
- Impaired GCR1 transcription resulted in defective inositol levels, vacuolar structure and autophagy in Saccharomyces cerevisiae.Current genetics · 2019Article
- Review
- The expression of PHO92 is regulated by Gcr1, and Pho92 is involved in glucose metabolism in Saccharomyces cerevisiae.Current genetics · 2014Article
- The multifaceted roles of metabolic enzymes in the Paracoccidioides species complex.Frontiers in microbiology · 2014Review
- Regulation of the hypoxic response in Candida albicans.Eukaryotic cell · 2010Article
- Transcriptional regulation of carbohydrate metabolism in the human pathogen Candida albicans.PLoS pathogens · 2009Article
- Coiled coil structures and transcription: an analysis of the S. cerevisiae coilome.Molecular genetics and genomics : MGG · 2007Article
- Regulation of glycolysis in Kluyveromyces lactis: role of KlGCR1 and KlGCR2 in glucose uptake and catabolism.Current genetics · 2004Article
- The unique features of glycolytic pathways in Archaea.The Biochemical journal · 2003Review
- Mutations in GCR1 affect SUC2 gene expression in Saccharomyces cerevisiae.Molecular genetics and genomics : MGG · 2003Article
- Microarray analysis of orthologous genes: conservation of the translational machinery across species at the sequence and expression level.Genome biology · 2003Article
- Rap1p requires Gcr1p and Gcr2p homodimers to activate ribosomal protein and glycolytic genes, respectively.Genetics · 2001Article
- Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns.Journal of bacteriology · 2000Article
- Transcript quantitation in total yeast cellular RNA using kinetic PCR.Nucleic acids research · 2000Article
- In vivo analysis of functional regions within yeast Rap1p.Molecular and cellular biology · 1999Article
- Genetic and physical interactions involving the yeast nuclear cap-binding complex.Molecular and cellular biology · 1999Article
- Osmotic stress-induced gene expression in Saccharomyces cerevisiae requires Msn1p and the novel nuclear factor Hot1p.Molecular and cellular biology · 1999Article
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1 author at 1 institution in 1 country.
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Abstract
In Saccharomyces cerevisiae, glycolysis enzymes constitute 30-60% of the soluble protein. GCR1 gene function is required for high-level glycolytic gene expression. In gcr1 mutant strains the levels of most glycolytic enzymes are between 2% and 10% of wild type. Binding sites for the global regulatory protein known as repressor activator protein 1 (RAP1)/general regulatory factor 1 (GRF1)/translation upstream factor (TUF) are found in close proximity to one or more CTTCC sequence motifs in the controlling region of GCR1-dependent genes. RAP1/GRF1/TUF-binding sites are known to be essential elements of upstream activating sequences that control expression of many glycolytic genes. In this report, I demonstrate that GCR1 encodes a DNA binding protein whose ability to bind DNA is dependent on the CTTCC sequence motif. This finding, in addition to the work of others, suggests that the GCR1 gene product and the RAP1/GRF1/TUF gene product act in concert to mediate high-level glycolytic gene expression.
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