ArticleNeurochemical research2009
An improved method to obtain a soluble nuclear fraction from embryonic brain tissue.
Article in Neurochemical research, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Decellularized corneal-based 3D scaffolds: methods decellularization, characterization, mechanical properties, and species source.Regenerative therapy · 2026Review
- Decellularization and Their Significance for Tissue Regeneration in the Era of 3D Bioprinting.ACS omega · 2024Review
- Development and Characterization of Acellular Caprine Choncal Cartilage Matrix for Tissue Engineering Applications.Cartilage · 2021Article
- Decellularization of fibroblast cell sheets for natural extracellular matrix scaffold preparation.Tissue engineering. Part C, Methods · 2015Article
- Native extracellular matrix: a new scaffolding platform for repair of damaged muscle.Frontiers in physiology · 2014Review
- Keeping an eye on decellularized corneas: a review of methods, characterization and applications.Journal of functional biomaterials · 2013Article
- An overview of tissue and whole organ decellularization processes.Biomaterials · 2011Review
- Rapid porcine corneal decellularization through the use of sodium N-lauroyl glutamate and supernuclease.Journal of tissue engineeringArticle
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Authors and funding
4 authors.
Funding
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Abstract
This paper describes modifications of the standard methods for obtaining a soluble nuclear fraction from embryonic brain tissue. The main improvements are: (1) the inclusion of a low speed centrifugation step to prevent the appearance of high density contaminants, (2) a sucrose density gradient to remove perinuclear mitochondria and ER membranes and (3) a protein extraction approach which significantly enhances protein yield. To demonstrate the effectiveness of the method, pellets were analyzed by light and electron microscopy and purity of the soluble extracts was immunologically tested. Finally, to illustrate the applicability of this approach, the induction of the transcription factor HIF-1 (hypoxia-inducible factor-1) was assessed by Western blot using soluble nuclear fractions and by immuno-electron microscopy using purified nuclear fractions, both obtained from the optic lobes of chick embryos. In conclusion, the procedure presently described appears to be reliable and convenient for obtaining a pure soluble nuclear fraction from a discrete amount of embryonic brain tissue.
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19462231What OpenQuestion holds
Registered trials
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