ArticlePloS one2009
Anode biofilm transcriptomics reveals outer surface components essential for high density current production in Geobacter sulfurreducens fuel cells.
Article in PloS one, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.
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Who cites it
135 citing papers in PubMed, 440 citations in OpenAlex.
- Overview of pathways and dynamic interactions of c-type cytochromes in Geobacter sulfurreducens.Biochemical Society transactions · 2026Review
- Comparative transcriptomics and scarless genome editing uncover a periplasmicApplied and environmental microbiology · 2026Article
- Expression and Engineering of Conductive Cytochrome Nanowires.bioRxiv : the preprint server for biology · 2026Article
- Nanostructured Hydrogels: A Method to Prevent Biofilms on Implantable Medical Devices.Gels (Basel, Switzerland) · 2026Review
- Comparative transcriptomics of closely relatedFrontiers in microbiology · 2026Article
- Relationship of electrochemical performance and biofilm development ofFrontiers in microbiology · 2026Article
- Evolution for enhanced extracellular electron transfer inFrontiers in microbiology · 2026Article
- Self-Powered Flexible Sensors: Recent Advances, Technological Breakthroughs, and Application Prospects.Sensors (Basel, Switzerland) · 2025Review
- Influence of support materials on the electroactive behavior, structure and gene expression of wild type and GSU1771-deficient mutant of Geobacter sulfurreducens biofilms.Environmental science and pollution research international · 2025Article
- The role of CsrA in controls the extracellular electron transfer and biofilm production inFrontiers in microbiology · 2025Article
- Electron transport across the cell envelope via multihemeFrontiers in chemistry · 2025Review
- Article
- Novel Insights on Extracellular Electron Transfer Networks in theMicroorganisms · 2024Article
- Article
- Widespread extracellular electron transfer pathways for charging microbial cytochrome OmcS nanowires via periplasmic cytochromes PpcABCDE.Nature communications · 2024Article
- Stimulating bioelectric generation and recovery of toxic metals through benthic microbial fuel cell driven by local sago (Cycas revoluta) waste.Environmental science and pollution research international · 2024Article
- Multi-heme cytochrome-mediated extracellular electron transfer by the anaerobic methanotroph 'Candidatus Methanoperedens nitroreducens'.Nature communications · 2023Article
- Bioenergy Generation and Phenol Degradation through Microbial Fuel Cells Energized by Domestic Organic Waste.Molecules (Basel, Switzerland) · 2023Article
- Biophotoelectrochemical process co-driven by dead microalgae and live bacteria.The ISME journal · 2023Article
- Nitrogen Fixation and Ammonium Assimilation Pathway Expression of Geobacter sulfurreducens Changes in Response to the Anode Potential in Microbial Electrochemical Cells.Applied and environmental microbiology · 2023Article
75 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The mechanisms by which Geobacter sulfurreducens transfers electrons through relatively thick (>50 microm) biofilms to electrodes acting as a sole electron acceptor were investigated. Biofilms of Geobacter sulfurreducens were grown either in flow-through systems with graphite anodes as the electron acceptor or on the same graphite surface, but with fumarate as the sole electron acceptor. Fumarate-grown biofilms were not immediately capable of significant current production, suggesting substantial physiological differences from current-producing biofilms. Microarray analysis revealed 13 genes in current-harvesting biofilms that had significantly higher transcript levels. The greatest increases were for pilA, the gene immediately downstream of pilA, and the genes for two outer c-type membrane cytochromes, OmcB and OmcZ. Down-regulated genes included the genes for the outer-membrane c-type cytochromes, OmcS and OmcT. Results of quantitative RT-PCR of gene transcript levels during biofilm growth were consistent with microarray results. OmcZ and the outer-surface c-type cytochrome, OmcE, were more abundant and OmcS was less abundant in current-harvesting cells. Strains in which pilA, the gene immediately downstream from pilA, omcB, omcS, omcE, or omcZ was deleted demonstrated that only deletion of pilA or omcZ severely inhibited current production and biofilm formation in current-harvesting mode. In contrast, these gene deletions had no impact on biofilm formation on graphite surfaces when fumarate served as the electron acceptor. These results suggest that biofilms grown harvesting current are specifically poised for electron transfer to electrodes and that, in addition to pili, OmcZ is a key component in electron transfer through differentiated G. sulfurreducens biofilms to electrodes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.