ArticleThe Journal of molecular diagnostics : JMD2009
A quantitative ELISA assay for the fragile x mental retardation 1 protein.
Article in The Journal of molecular diagnostics : JMD, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 59 citations in OpenAlex.
- Electroretinographic Findings in Fragile X, Premutation, and Controls: A Study of Biomarker Correlations.International journal of molecular sciences · 2025Article
- Optimization, validation and initial clinical implications of a Luminex-based immunoassay for the quantification of Fragile X Protein from dried blood spots.Scientific reports · 2022Article
- Fragile X Mental Retardation Protein and Cerebral Expression of Metabotropic Glutamate Receptor Subtype 5 in Men with Fragile X Syndrome: A Pilot Study.Brain sciences · 2022Article
- FMRP Levels in Human Peripheral Blood Leukocytes Correlates with Intellectual Disability.Diagnostics (Basel, Switzerland) · 2021Article
- Elevated FMR1-mRNA and lowered FMRP - A double-hit mechanism for psychiatric features in men with FMR1 premutations.Translational psychiatry · 2020Article
- Association between IQ and FMR1 protein (FMRP) across the spectrum of CGG repeat expansions.PloS one · 2019Article
- Recent advances in assays for the fragile X-related disorders.Human genetics · 2017Review
- Review
- Modeling fragile X syndrome in the Fmr1 knockout mouse.Intractable & rare diseases research · 2014Review
- FXTAS in an unmethylated mosaic male with fragile X syndrome from Chile.Clinical genetics · 2014Article
- Association between macroorchidism and intelligence in FMR1 premutation carriers.American journal of medical genetics. Part A · 2014Article
- CNS expression of murine fragile X protein (FMRP) as a function of CGG-repeat size.Human molecular genetics · 2014Article
- The challenges of clinical trials in fragile X syndrome.Psychopharmacology · 2014Review
- Enhanced asynchronous Ca(2+) oscillations associated with impaired glutamate transport in cortical astrocytes expressing Fmr1 gene premutation expansion.The Journal of biological chemistry · 2013Article
- A quantitative homogeneous assay for fragile X mental retardation 1 protein.Journal of neurodevelopmental disorders · 2013Article
- Influence of the fragile X mental retardation (FMR1) gene on the brain and working memory in men with normal FMR1 alleles.NeuroImage · 2013Article
- Early mitochondrial abnormalities in hippocampal neurons cultured from Fmr1 pre-mutation mouse model.Journal of neurochemistry · 2012Article
- Clustered burst firing in FMR1 premutation hippocampal neurons: amelioration with allopregnanolone.Human molecular genetics · 2012Article
- Abnormal dendrite and spine morphology in primary visual cortex in the CGG knock-in mouse model of the fragile X premutation.Epilepsia · 2012Article
- A fragile X sibship from a consanguineous family with a compound heterozygous female and partially methylated full mutation male.American journal of medical genetics. Part A · 2012Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
Non-coding (CGG-repeat) expansions in the fragile X mental retardation 1 (FMR1) gene result in a spectrum of disorders involving altered neurodevelopment (fragile X syndrome), neurodegeneration (late-onset fragile X-associated tremor/ataxia syndrome), or primary ovarian insufficiency. While reliable and quantitative assays for the number of CGG repeats and FMR1 mRNA levels are now available, there has been no scalable, quantitative assay for the FMR1 protein (FMRP) in non-transformed cells. Using a combination of avian and murine antibodies to FMRP, we developed a sensitive and highly specific sandwich enzyme-linked immunosorbent assay (ELISA) for FMRP in peripheral blood lymphocytes. This ELISA method is capable of quantifying FMRP levels throughout the biologically relevant range of protein concentrations and is specific for the intact FMRP protein. Moreover, the ELISA is well-suited for replicate protein determinations across serial dilutions in non-transformed cells and is readily scalable for large sample numbers. The FMRP ELISA is potentially a powerful tool in expanding our understanding of the relationship between FMRP levels and the various FMR1-associated clinical phenotypes.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.