Evidence map›Paper›PMID 19459645›Full record

ArticleJournal of medicinal chemistry2009

Nicotinic agonist binding site mapped by methionine- and tyrosine-scanning coupled with azidochloropyridinyl photoaffinity labeling.

Motohiro Tomizawa, Todd T Talley, John F Park, David Maltby, Katalin F Medzihradszky, Kathleen A Durkin, Jose M Cornejo-Bravo, Alma L Burlingame, John E Casida, Palmer Taylor

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of medicinal chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
3.9field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 19 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 1 country.

Motohiro TomizawaEnvironmental Chemistry and Toxicology Laboratory, Department of Environmental Science, Policy and Management, University of California, Berkeley, California 94720-3112, USA.
Todd T Talley
John F Park
David Maltby
Katalin F Medzihradszky
Kathleen A Durkin
Jose M Cornejo-Bravo
Alma L Burlingame
John E Casida
Palmer Taylor
University of Montana · USUniversity of California, San Francisco · USUniversity of California, Berkeley · US

Funding

YEAST THREONINE &SERINE KINASESP41RR001614 · NCRR · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI SZOKA, FRANCIS C. · 1985 to 2011
$24.1M
KINETICS OF DRUG MACROMOLECULE COMPLEX FORMATIONR37GM018360 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI TAYLOR, PALMER WILLIAM · 1999 to 2008
$5.7M
CHOLINERGIC INSECTICIDE TOXICOLOGYR01ES008424 · NIEHS · UNIVERSITY OF CALIFORNIA BERKELEY · PI CASIDA, JOHN E · 1998 to 2006
$1.5M
PURCHASE OF LTQ FT-ICR HYBRID MASS SPECTROMETER: (HEI) PROTEOMICSS10RR019934 · NCRR · UNIVERSITY OF CALIFORNIA SAN FRANCISCO · PI BURLINGAME, ALMA L · 2004 to 2004
$1.0M
Alpha Neurotoxin Probes of Nicotinic AChRF32NS043063 · NINDS · UNIVERSITY OF CALIFORNIA SAN DIEGO · PI TALLEY, TODD T · 2002 to 2004
$134k
NCRR NIH HHS P41 RR001614NCRR NIH HHS RR001614NCRR NIH HHS RR015084NCRR NIH HHS RR019934NCRR NIH HHS S10 RR019934NIEHS NIH HHS R01 ES008424NIEHS NIH HHS R01 ES08424NIGMS NIH HHS R37 GM018360NIGMS NIH HHS R37-GM18360NINDS NIH HHS F32 NS043063NINDS NIH HHS U01-NS05846
6 · The paper itself

Abstract

Agonists activating nicotinic acetylcholine receptors (nAChR) include potential therapeutic agents and also toxicants such as epibatidine and neonicotinoid insecticides with a chloropyridinyl substituent. Nicotinic agonist interactions with mollusk (Aplysia californica) acetylcholine binding protein, a soluble surrogate of the nAChR extracellular domain, are precisely defined by scanning with 17 methionine and tyrosine mutants within the binding site by photoaffinity labeling with 5-azido-6-chloropyridin-3-yl probes that have similar affinities to their nonazido counterparts. Methionine and tyrosine are the only residues found derivatized, and their reactivity exquisitely depends on the direction of the azido moiety and its apposition to the reactive amino acid side chains.

Indexed as

AnimalsAplysiaAzidesBinding SitesComputer SimulationMass SpectrometryMethionineModels, ChemicalMolecular StructurePhotoaffinity LabelsPyridinesReceptors, NicotinicTyrosineAzidesMethioninePhotoaffinity LabelsPyridinesReceptors, NicotinicTyrosine

Identifiers

PMID19459645
PMCPMC2748672
OpenAlexW2026878016

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.