ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2009
Epitope-tagging Math5 and Pou4f2: new tools to study retinal ganglion cell development in the mouse.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Key transcription factors influence the epigenetic landscape to regulate retinal cell differentiation.Nucleic acids research · 2023Article
- Genetic control of retinal ganglion cell genesis.Cellular and molecular life sciences : CMLS · 2021Review
- Single cell transcriptomics reveals lineage trajectory of retinal ganglion cells in wild-type and Atoh7-null retinas.Nature communications · 2021Article
- Single Cell Transcriptomic Analyses Reveal the Impact of bHLH Factors on Human Retinal Organoid Development.Frontiers in cell and developmental biology · 2021Article
- Two new genetically modified mouse alleles labeling distinct phases of retinal ganglion cell development by fluorescent proteins.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Elevated expression of human bHLH factor ATOH7 accelerates cell cycle progression of progenitors and enhances production of avian retinal ganglion cells.Scientific reports · 2018Article
- The dynamics of native Atoh7 protein expression during mouse retinal histogenesis, revealed with a new antibody.Gene expression patterns : GEP · 2018Article
- Temporal expression of CD184(CXCR4) and CD171(L1CAM) identifies distinct early developmental stages of human retinal ganglion cells in embryonic stem cell derived retina.Experimental eye research · 2017Article
- Novel Roles and Mechanism for Krüppel-like Factor 16 (KLF16) Regulation of Neurite Outgrowth and Ephrin Receptor A5 (EphA5) Expression in Retinal Ganglion Cells.The Journal of biological chemistry · 2016Article
- Two transcription factors, Pou4f2 and Isl1, are sufficient to specify the retinal ganglion cell fate.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Transcriptome of Atoh7 retinal progenitor cells identifies new Atoh7-dependent regulatory genes for retinal ganglion cell formation.Developmental neurobiology · 2014Article
- Birth of cone bipolar cells, but not rod bipolar cells, is associated with existing RGCs.PloS one · 2014Article
- Onecut1 is essential for horizontal cell genesis and retinal integrity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Reprogramming amacrine and photoreceptor progenitors into retinal ganglion cells by replacing Neurod1 with Atoh7.Development (Cambridge, England) · 2013Article
- Math5 defines the ganglion cell competence state in a subpopulation of retinal progenitor cells exiting the cell cycle.Developmental biology · 2012Article
- Onecut 1 and Onecut 2 are potential regulators of mouse retinal development.The Journal of comparative neurology · 2012Article
- The deletion of Math5 disrupts retinal blood vessel and glial development in mice.Experimental eye research · 2012Article
- Adult mice transplanted with embryonic retinal progenitor cells: new approach for repairing damaged optic nerves.Molecular vision · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
Abstract
Although immunological detection of proteins is used extensively in retinal development, studies are often impeded because antibodies against crucial proteins cannot be generated or are not readily available. Here, we overcome these limitations by constructing genetically engineered alleles for Math5 and Pou4f2, two genes required for retinal ganglion cell (RGC) development. Sequences encoding a peptide epitope from haemagglutinin (HA) were added to Math5 or Pou4f2 in frame to generate Math5(HA) and Pou4f2(HA) alleles. We demonstrate that the tagged alleles recapitulated the wild-type expression patterns of the two genes, and that the tags did not interfere with the function of the cognate proteins. In addition, by co-staining, we found that Math5 and Pou4f2 were transiently co-expressed in newly born RGCs, unequivocally demonstrating that Pou4f2 is immediately downstream of Math5 in RGC formation. The epitope-tagged alleles provide new and useful tools for analyzing gene regulatory networks underlying RGC development.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.