ArticleBiotechnology and bioengineering2009
Flow-dependent entrapment of large bioparticles in porous process media.
Article in Biotechnology and bioengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Resilient performance indicator virus for membrane filtration considering surface charge, hydrophobicity, and morphology.Applied and environmental microbiology · 2026Article
- Developing a process of lentivirus purification from recombinant fluids using peptide affinity ligands.Bioengineering & translational medicine · 2025Article
- Navigating the Purification Process: Maintaining the Integrity of Replication-Competent Enveloped Viruses.Vaccines · 2025Review
- Purification of Adeno-Associated Virus (AAV) Serotype 2 fromMembranes · 2022Article
- Lentiviral Vector Purification Using Nanofiber Ion-Exchange Chromatography.Molecular therapy. Methods & clinical development · 2019Article
- Significance of Brownian Motion for Nanoparticle and Virus Capture in Nanocellulose-Based Filter Paper.Membranes · 2018Article
- Characterizing the impact of pressure on virus filtration processes and establishing design spaces to ensure effective parvovirus removal.Biotechnology progress · 2017Article
- Modeling of dispersion in a polymeric chromatographic monolith.Journal of chromatography. A · 2012Article
- Downstream processing of cell culture-derived virus particles.Expert review of vaccines · 2011Review
- Effect of bioparticle size on dispersion and retention in monolithic and perfusive beds.Journal of chromatography. A · 2010Article
- Downstream Processing Technologies/Capturing and Final Purification : Opportunities for Innovation, Change, and Improvement. A Review of Downstream Processing Developments in Protein Purification.Advances in biochemical engineering/biotechnologyReview
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
The need for purification of biomolecules extends to larger bioparticles as well. For example, virus purification is required for production of many vaccines and gene delivery vectors, and understanding virus removal in porous media is also important in downstream processing of therapeutic proteins and in purification of water in soils. A convective entrapment mechanism for retention of large bioparticles is discussed here based on retention of such bioparticles in pore constrictions at high enough flow rates, even under non-binding conditions. A simple equation to predict whether such entrapment is expected to occur in a given system is derived based on a Péclet number that is proportional to the flow rate and to the cube of the bioparticle diameter. To test the theory, adenovirus was spiked onto chromatographic beds. As expected from the theory, under non-interacting conditions a progressively larger amount of virus becomes trapped with increasing flow rate. The entrapment is reversible upon flow rate reduction, which, within the proposed model, is based on the possibility of diffusive escape from pore constrictions. This mechanism can be exploited for virus purification or removal, and the theory is also consistent with the anecdotal evidence that monoliths and membranes are more difficult to clean than conventional chromatographic beds, especially at high flow rates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.