ArticleMolecular imaging and biology
Death and proliferation time course of stem cells transplanted in the myocardium.
Article in Molecular imaging and biology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- Inhibition of Gap Junction Formation Prior to Implantation of Bone Marrow-Derived Mesenchymal Cells Improves Function in the Ischemic Myocardium.International journal of molecular sciences · 2023Article
- 7-T MRI tracking of mesenchymal stromal cells after lung injection in a rat model.European radiology experimental · 2020Article
- Y-27632 preconditioning enhances transplantation of human-induced pluripotent stem cell-derived cardiomyocytes in myocardial infarction mice.Cardiovascular research · 2019Article
- Transected Tendon Treated with a New Fibrin Sealant Alone or Associated with Adipose-Derived Stem Cells.Cells · 2019Article
- Noninvasive Monitoring of Allogeneic Stem Cell Delivery with Dual-Modality Imaging-Visible Microcapsules in a Rabbit Model of Peripheral Arterial Disease.Stem cells international · 2019Article
- Pluripotent Stem Cell Derived Cardiac Cells for Myocardial Repair.Journal of visualized experiments : JoVE · 2017Article
- Bioengineering methods for myocardial regeneration.Advanced drug delivery reviews · 2016Review
- Exogenous Nitric Oxide Protects Human Embryonic Stem Cell-Derived Cardiomyocytes against Ischemia/Reperfusion Injury.Oxidative medicine and cellular longevity · 2016Article
- Cell-based therapy for heart failure in rat: double thoracotomy for myocardial infarction and epicardial implantation of cells and biomatrix.Journal of visualized experiments : JoVE · 2014Article
- Clinical imaging in regenerative medicine.Nature biotechnology · 2014Review
- Cobalt protoporphyrin pretreatment protects human embryonic stem cell-derived cardiomyocytes from hypoxia/reoxygenation injury in vitro and increases graft size and vascularization in vivo.Stem cells translational medicine · 2014Article
- The current status of iPS cells in cardiac research and their potential for tissue engineering and regenerative medicine.Stem cell reviews and reports · 2014Article
- Considerations for pre-clinical models and clinical trials of pluripotent stem cell-derived cardiomyocytes.Stem cell research & therapy · 2014Review
- Imaging Cell Therapy for Myocardial Regeneration.Current cardiovascular imaging reports · 2012Article
- Positron emission tomography reporter genes and reporter probes: gene and cell therapy applications.Theranostics · 2012Article
- A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart.Regenerative medicine · 2011Article
- A fibrin patch-based enhanced delivery of human embryonic stem cell-derived vascular cell transplantation in a porcine model of postinfarction left ventricular remodeling.Stem cells (Dayton, Ohio) · 2011Article
- Embryonic stem cell-derived cardiomyocyte heterogeneity and the isolation of immature and committed cells for cardiac remodeling and regeneration.Stem cells international · 2011Article
- Utility of dual-modality bioluminescence and MRI in monitoring stem cell survival and impact on post myocardial infarct remodeling.Academic radiology · 2011Article
- Current status of stem cell therapy in heart failure.Current cardiology reports · 2010Review
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
purposeNoninvasive positron emission tomography (PET) imaging of reporter gene is combined with quantitative real-time polymerase reverse transcription (RT-PCR) method to study the time course of death and proliferation of stem cells transplanted in the myocardium.
methodsMale murine embryonic stem cells (ESCs) were stably transfected with a mutant version of herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) reporter gene; 5 x 10(6) such cells were injected into the myocardium of female athymic rats. While the transplanted cells was monitored by in vivo 9-(4-[F-18]fluoro-3-hydroxymethylbutyl)guanine ([F-18]FHBG) PET imaging of the heart, their absolute number was estimated by RT-PCR from hearts harvested at 3-5 h, 24 h, days 4, 7, and 14 after transplantation.
results(1) Forty percent of injected cells were retained in the heart while majority of injected cells were lost within a few hours after injection. Cell death was peaked at 24 h when 18% of donor cells retained in the heart were dead. (2) The substantial cell loss was reversed by significant proliferation of ESCs. This led to the recovery of cell number to 3.4 million (70% of injected dose) at day 4 and first visual observation of in vivo [F-18] signal in the heart. (3) A robust correlation (R (2) = 0.9) between percent of injected dose per gram of tissue derived from in vivo PET signal and the number of donor cells estimated by RT-PCR was revealed.
conclusionsThe time course of transplanted stem cells surviving in the heart reveals a process of substantial cell loss within 24 h of injection and subsequent recovery of cell number through proliferation. Such proliferation can be noninvasively monitored by reporter gene imaging.
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