Evidence map›Paper›PMID 19458948›Full record

ArticleEuropean biophysics journal : EBJ2009

Intermittent contact mode AFM investigation of native plasma membrane of Xenopus laevis oocyte.

Francesco Orsini, M Santacroce, P Arosio, M Castagna, C Lenardi, G Poletti, F V Sacchi

Abstract read
PubMed Publisher
In one paragraph

Article in European biophysics journal : EBJ, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Francesco OrsiniGiovanni Esposito Institute of General Physiology and Biological Chemistry, University of Milan, Via Trentacoste 2, 20134, Milan, Italy. francesco.orsini@unimi.it
M Santacroce
P Arosio
M Castagna
C Lenardi
G Poletti
F V Sacchi

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Intermittent contact mode atomic force microscopy (AFM) was used to visualize the native plasma membrane of Xenopus laevis oocytes. Oocyte membranes were purified via ultracentrifugation on a sucrose gradient and adsorbed on mica leaves. AFM topographs and the corresponding phase images allowed for visualization and identification of both oocyte plasma membrane patches and pure lipid bilayer regions with a height of about 5 nm within membrane patches. The quantitative analysis showed a normal distribution for the lateral dimension and height of the protein complexes centered on 16.7 +/- 0.2 nm (mean +/- SE, n = 263) and 5.4 +/- 0.1 nm (n = 262), respectively. The phase signal, providing material-dependent information, allowed for the recognition of structural features observed in AFM topographs.

Indexed as

Xenopus laevisAdsorptionAluminum SilicatesAnimalsCell MembraneCrystallography, X-RayFemaleLipid BilayersMembrane ProteinsMicroscopy, Atomic ForceOocytesSucroseTime FactorsUltracentrifugationAluminum SilicatesLipid BilayersMembrane ProteinsmicaSucrose

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.