ArticleGene therapy2009
Visualization of in vivo electroporation-mediated transgene expression in experimental tumors by optical and magnetic resonance imaging.
Article in Gene therapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
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- Recent progress of emitting long-wavelength carbon dots and their merits for visualization tracking, target delivery and theranostics.Theranostics · 2023Review
- Advanced strategies for nucleic acids and small-molecular drugs in combined anticancer therapy.International journal of biological sciences · 2023Review
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Dual-Effect of Magnetic Resonance Imaging Reporter Gene in Diagnosis and Treatment of Hepatocellular Carcinoma.International journal of nanomedicine · 2020Article
- Wearable Devices for Single-Cell Sensing and Transfection.Trends in biotechnology · 2019Review
- MR molecular imaging of HCC employing a regulated ferritin gene carried by a modified polycation vector.International journal of nanomedicine · 2019Article
- Ferritin-EGFP Chimera as an Endogenous Dual-Reporter for Both Fluorescence and Magnetic Resonance Imaging in Human Glioma U251 Cells.Tomography (Ann Arbor, Mich.) · 2017Article
- Overexpression of the MRI Reporter Genes Ferritin and Transferrin Receptor Affect Iron Homeostasis and Produce Limited Contrast in Mesenchymal Stem Cells.International journal of molecular sciences · 2015Article
- Magnetic Resonance Imaging Tracking of Graft Survival in the Infarcted Heart: Iron Oxide Particles Versus Ferritin Overexpression Approach.Journal of cardiovascular pharmacology and therapeutics · 2014Article
- Imaging tumor growth non-invasively using expression of MagA or modified ferritin subunits to augment intracellular contrast for repetitive MRI.Molecular imaging and biology · 2014Article
- Construction and identification of the adenoviral vector with dual reporter gene for multimodality molecular imaging.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2013Article
- MRI reporter genes: applications for imaging of cell survival, proliferation, migration and differentiation.NMR in biomedicine · 2013Review
- Quantification of HSV-1-mediated expression of the ferritin MRI reporter in the mouse brain.Gene therapy · 2013Article
- Quantification of MRI signal of transgenic grafts overexpressing ferritin in murine myocardial infarcts.NMR in biomedicine · 2012Article
- In vivo molecular imaging and histological analysis of changes induced by electric pulses used for plasmid DNA electrotransfer to the skin: a study in a dorsal window chamber in mice.The Journal of membrane biology · 2012Article
- Is there a path beyond BOLD? Molecular imaging of brain function.NeuroImage · 2012Review
- Muscle gene electrotransfer is increased by the antioxidant tempol in mice.Gene therapy · 2012Article
- MRI study on reversible and irreversible electroporation induced blood brain barrier disruption.PloS one · 2012Article
- Diffusion-weighted MRI for verification of electroporation-based treatments.The Journal of membrane biology · 2011Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In vivo electroporation (EP) is an efficient method for effective gene transfer and is highly expected for application in anticancer gene therapy. Non-invasive monitoring of gene transfer/expression is critical for optimal gene therapy. Here we report in vivo optical and high-field magnetic resonance imaging (MRI) of EP-mediated transgene expression in a tumor model. Initially, we observed spatio-temporal change in in vivo EP-mediated transgene expression by optical imaging using red fluorescence protein (RFP) as a reporter gene. Next, we constructed a dual-reporter plasmid carrying a gene-encoding MRI reporter ferritin heavy chain and RFP gene to visualize the intratumoral transgene expression by dual modality. Cells transfected with this plasmid showed lower signal intensity on in vitro T(2)-weighted cellular MRI and quantitatively increased the transverse relaxation rate (1/T(2)) compared with control cells. After conducting in vivo EP in an experimental tumor, the plasmid-injected region showed both fluorescent emissions in optical imaging and detectably lowered signal on T(2)-weighted MRI. The correlative immunohistological findings confirmed that both the reporter transgenes were co-expressed in this region. Thus, our strategy provides a platform for evaluating EP-mediated cancer gene therapy easily and safely without administering contrast agent or substrate.
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