ArticleInvestigative ophthalmology & visual science2009
Association of superoxide anions with retinal pigment epithelial cell apoptosis induced by mononuclear phagocytes.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 24 citations in OpenAlex.
- Fundus blood flow density changes in the smoking population by artificial intelligence-based optical coherence tomography angiography.International journal of ophthalmology · 2025Article
- Selenium prevents interferon-gamma induced activation of TRPM2 channel and inhibits inflammation, mitochondrial oxidative stress, and apoptosis in microglia.Metabolic brain disease · 2021Article
- The role of lymphocytes and phagocytes in age-related macular degeneration (AMD).Cellular and molecular life sciences : CMLS · 2020Review
- Targeting the P2X7 Receptor in Age-Related Macular Degeneration.Vision (Basel, Switzerland) · 2017Review
- Activated monocytes resist elimination by retinal pigment epithelium and downregulate their OTX2 expression via TNF-α.Aging cell · 2017Article
- Inhibition of the oxidative stress-induced miR-23a protects the human retinal pigment epithelium (RPE) cells from apoptosis through the upregulation of glutaminase and glutamine uptake.Molecular biology reports · 2016Article
- N-Acetylcysteine Amide Protects Against Oxidative Stress-Induced Microparticle Release From Human Retinal Pigment Epithelial Cells.Investigative ophthalmology & visual science · 2016Article
- Nuclear Factor (Erythroid-Derived)-Related Factor 2-Associated Retinal Pigment Epithelial Cell Protection under Blue Light-Induced Oxidative Stress.Oxidative medicine and cellular longevity · 2016Article
- Inflammatory cytokines protect retinal pigment epithelial cells from oxidative stress-induced death.PloS one · 2013Article
- Retinal flavoprotein fluorescence correlates with mitochondrial stress, apoptosis, and chemokine expression.Experimental eye research · 2011Article
- MCP-1-activated monocytes induce apoptosis in human retinal pigment epithelium.Investigative ophthalmology & visual science · 2011Article
- Activation of P2X receptors induces apoptosis in human retinal pigment epithelium.Investigative ophthalmology & visual science · 2011Article
- SOD2 contributes to anti-oxidative capacity in rabbit corneal endothelial cells.Molecular vision · 2011Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeOxidative stress of the retinal pigment epithelium by reactive oxygen species and monocytic infiltration have been implicated in age-related macular degeneration. The purpose of this study was to determine the role of superoxide anions (O(2)(-)) in mononuclear phagocyte-induced RPE apoptosis.
methodsMouse RPE cell cultures were established from wild-type and heterozygous superoxide dismutase 2-knockout (Sod2(+/-)) mice. The intracellular reactive oxygen species, O(2)(-) and hydrogen peroxide, were measured by using dihydroethidium assay and 5-(and 6)-chloromethyl-2',7'-dichlorodihydrofluorescence diacetate, acetyl ester assay, respectively. RPE apoptosis was evaluated by Hoechst staining and terminal deoxynucleotidyltransferase dUTP nick-end labeling assay. Changes in mitochondrial membrane potential were detected by 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethylbenzimidazolylcarbocyanine iodide dye. Activated caspases and caspase-3 were detected in situ by FITC-VAD-fmk staining and caspase-3 substrate, respectively.
resultsMononuclear phagocytes and interferon-gamma-activated mononuclear phagocytes induced the production of intracellular RPE O(2)(-), a decrease in RPE mitochondrial membrane potential, caspase activation, and apoptosis of mouse RPE cells. All theses changes were significantly enhanced in the Sod2(+/-) RPE cells. Activated mononuclear phagocytes induced more of these oxidative and apoptotic changes in RPE cells than did unstimulated mononuclear phagocytes.
conclusionsThe authors have shown that the decreased expression of SOD2 and increased superoxide production correlate with RPE apoptosis induced by unstimulated and activated mononuclear phagocytes. The authors suggest that elevated O(2)(-) levels due to genetic abnormalities of SOD2 or immunologic activation of mononuclear phagocytes lead to greater levels of RPE apoptosis. The present study could serve as a useful model to characterize RPE/phagocyte interaction in AMD and other retinal diseases.
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