ArticleMolecular biology of the cell2009
Dephosphorylation of major sperm protein (MSP) fiber protein 3 by protein phosphatase 2A during cell body retraction in the MSP-based amoeboid motility of Ascaris sperm.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 29 citations in OpenAlex.
- Released ATP Mediates Spermatozoa Chemotaxis Promoted by Uterus-Derived Factor (UDF) inInternational journal of molecular sciences · 2022Article
- Parasite protein phosphatases: biological function, virulence, and host immune evasion.Parasitology research · 2021Review
- The intrinsically disordered protein SPE-18 promotes localized assembly of MSP inDevelopment (Cambridge, England) · 2021Article
- Cytoskeletal variations in an asymmetric cell division support diversity in nematode sperm size and sex ratios.Development (Cambridge, England) · 2017Article
- Article
- Extending the molecular clutch beyond actin-based cell motility.New journal of physics · 2014Article
- Cytosolic Ca(2+) as a multifunctional modulator is required for spermiogenesis in Ascaris suum.Protein & cell · 2013Article
- Calcium signaling surrounding fertilization in the nematode Caenorhabditis elegans.Cell calcium · 2013Review
- Transformation: how do nematode sperm become activated and crawl?Protein & cell · 2012Review
- Identification of Potential Glycoprotein Biomarkers in Estrogen Receptor Positive (ER+) and Negative (ER-) Human Breast Cancer Tissues by LC-LTQ/FT-ICR Mass Spectrometry.Journal of Cancer · 2012Article
- SPE-44 implements sperm cell fate.PLoS genetics · 2012Article
- Sperm development and motility are regulated by PP1 phosphatases in Caenorhabditis elegans.Genetics · 2012Article
- Reconstitution of amoeboid motility in vitro identifies a motor-independent mechanism for cell body retraction.Current biology : CB · 2011Article
- Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad.BMC genomics · 2011Article
- New insights into the mechanism of fertilization in nematodes.International review of cell and molecular biology · 2011Review
- Spermatogenesis-defective (spe) mutants of the nematode Caenorhabditis elegans provide clues to solve the puzzle of male germline functions during reproduction.Developmental dynamics : an official publication of the American Association of Anatomists · 2010Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
The crawling movement of nematode sperm requires coordination of leading edge protrusion with cell body retraction, both of which are powered by modulation of a cytoskeleton based on major sperm protein (MSP) filaments. We used a cell-free in vitro motility system in which both protrusion and retraction can be reconstituted, to identify two proteins involved in cell body retraction. Pharmacological and depletion-add back assays showed that retraction was triggered by a putative protein phosphatase 2A (PP2A, a Ser/Thr phosphatase activated by tyrosine dephosphorylation). Immunofluorescence showed that PP2A was present in the cell body and was concentrated at the base of the lamellipod where the force for retraction is generated. PP2A targeted MSP fiber protein 3 (MFP3), a protein unique to nematode sperm that binds to the MSP filaments in the motility apparatus. Dephosphorylation of MFP3 caused its release from the cytoskeleton and generated filament disassembly. Our results suggest that interaction between PP2A and MFP3 leads to local disassembly of the MSP cytoskeleton at the base of the lamellipod in sperm that in turn pulls the trailing cell body forward.
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