ArticleAmerican journal of physiology. Renal physiology2009
PP2B-dependent NO production in the medullary thick ascending limb during diabetes.
Article in American journal of physiology. Renal physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 22 citations in OpenAlex.
- Acclimation to a High-Salt Diet Is Sex Dependent.Journal of the American Heart Association · 2022Article
- Mechanisms of decreased tubular flow-induced nitric oxide in Dahl salt-sensitive rat thick ascending limbs.American journal of physiology. Renal physiology · 2021Article
- Hydroxyurea improves nitric oxide bioavailability in humanized sickle cell mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Article
- Sensing of tubular flow and renal electrolyte transport.Nature reviews. Nephrology · 2020Review
- Loss of circadian geneAmerican journal of physiology. Renal physiology · 2020Article
- Calcineurin-Mediated Hippocampal Inflammatory Alterations in Streptozotocin-Induced Model of Dementia.Molecular neurobiology · 2020Article
- Reactive species balance via GTP cyclohydrolase I regulates glioblastoma growth and tumor initiating cell maintenance.Neuro-oncology · 2018Article
- Deep Phosphoproteomic Measurements Pinpointing Drug Induced Protective Mechanisms in Neuronal Cells.Frontiers in physiology · 2016Article
- Calcineurin and Sorting-Related Receptor with A-Type Repeats Interact to Regulate the Renal Na⁺-K⁺-2Cl⁻ Cotransporter.Journal of the American Society of Nephrology : JASN · 2016Article
- Interaction between NO synthase and NADPH oxidase in control of sodium transport by the renal thick ascending limb during diabetes.Acta physiologica (Oxford, England) · 2013Article
- Renal collecting duct NOS1 maintains fluid-electrolyte homeostasis and blood pressure.Hypertension (Dallas, Tex. : 1979) · 2013Article
- On the selectivity of neuronal NOS inhibitors.British journal of pharmacology · 2013Article
- Salt sensitivity of nitric oxide generation and blood pressure in mice with targeted knockout of the insulin receptor from the renal tubule.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Changing standard chow diet promotes vascular NOS dysfunction in Dahl S rats.American journal of physiology. Regulatory, integrative and comparative physiology · 2012Article
- Distinct actions of endothelin A-selective versus combined endothelin A/B receptor antagonists in early diabetic kidney disease.The Journal of pharmacology and experimental therapeutics · 2011Article
- Dynamin activates NO production in rat renal inner medullary collecting ducts via protein-protein interaction with NOS1.American journal of physiology. Renal physiology · 2011Article
- Endothelin, nitric oxide, and reactive oxygen species in diabetic kidney disease.Contributions to nephrology · 2011Review
- Sex differences in acute ANG II-mediated hemodynamic responses in mice.American journal of physiology. Regulatory, integrative and comparative physiology · 2010Article
- Protein kinase C-dependent NAD(P)H oxidase activation induced by type 1 diabetes in renal medullary thick ascending limb.Hypertension (Dallas, Tex. : 1979) · 2010Article
- Effect of high soy diet on the cerebrovasculature and endothelial nitric oxide synthase in the ovariectomized rat.Vascular pharmacologyArticle
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
Calcineurin (PP2B) has recently been shown to be upregulated in the medullary thick ascending limb (mTAL) during diabetes. The mTAL expresses all three isoforms of nitric oxide synthase (NOS), which are subject to phosphoregulation and represent substrates for PP2B. Therefore, we hypothesized that diabetes induces PP2B-dependent upregulation of NOS activity and NO production in the mTAL. Three weeks after injection of streptozotocin (STZ rats) or vehicle (sham rats), mTAL suspensions were prepared for use in functional and biochemical assays. PP2B activity and expression were increased in mTALs from STZ rats compared with sham. Nitrite production was significantly reduced in mTALs from STZ rats compared with sham. However, incubation with the free radical scavenger, tempol, unmasked a significant increase in nitrite production by mTALs from STZ rats. Inhibition of PP2B attenuated the increase in nitrite production and NOS activity evident in mTALs from STZ rats. Analysis of specific NOS isoform activity revealed increased NOS1 and NOS2 activities in mTALs from STZ rats. All three NOS isoform activities were regulated in a PP2B-dependent manner. Western blot analysis detected no differences in NOS isoform expression, although phosphorylation of pThr(495)-NOS3 was significantly reduced in mTALs from STZ rats. Phosphorylation of pSer(852)-NOS1, pSer(633)-NOS3, and pSer(1177)-NOS3 was similar in mTALs from STZ and sham rats. Inhibition of PP2B did not alter the phosphorylation of NOS1 or NOS3 at known sites. In conclusion, while NO bioavailability in mTALs is reduced during diabetes, free radical scavenging with tempol unmasks increased NO production that involves PP2B-dependent activation of NOS1 and NOS2.
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