ArticleThe Journal of biological chemistry2009
Loss of alpha-catenin decreases the strength of single E-cadherin bonds between human cancer cells.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
37 citing papers in PubMed, 59 citations in OpenAlex.
- Adhesion-driven rigidity transition decoupled from density-driven jamming triggers epithelial organization in embryonic tissues.Nature physics · 2026Article
- Alpha T-catenin: a crucial tumor suppressor in cancer pathogenesis.Journal of molecular histology · 2024Review
- Engineering the Interactions of Classical Cadherin Cell-Cell Adhesion Proteins.Journal of immunology (Baltimore, Md. : 1950) · 2023Review
- The Role of CTNNA1 in Malignancies: An Updated Review.Journal of Cancer · 2023Review
- α-Catenin links integrin adhesions to F-actin to regulate ECM mechanosensing and rigidity dependence.The Journal of cell biology · 2022Article
- Genome-wide DNA methylation pattern in systemic sclerosis microvascular endothelial cells: Identification of epigenetically affected key genes and pathways.Journal of scleroderma and related disorders · 2022Article
- Inside-out regulation of E-cadherin conformation and adhesion.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Relationship Between Protein S-Nitrosylation and Human Diseases: A Review.Neurochemical research · 2020Review
- Single-molecule studies of classical and desmosomal cadherin adhesion.Current opinion in biomedical engineering · 2019Article
- Article
- Recurrent Extradural Myxopapillary Ependymoma With Oligometastatic Spread.Frontiers in oncology · 2019Article
- αE-catenin is a candidate tumor suppressor for the development of E-cadherin-expressing lobular-type breast cancer.The Journal of pathology · 2018Article
- Cell-cell adhesion interface: orthogonal and parallel forces from contraction, protrusion, and retraction.F1000Research · 2018Review
- Intracellular Domain Contacts Contribute to Ecadherin Constitutive Dimerization in the Plasma Membrane.Journal of molecular biology · 2017Article
- Epidermal growth factor receptor targeting alters gene expression and restores the adhesion function of cancerous cells as measured by single cell force spectroscopy.Molecular and cellular biochemistry · 2016Article
- CTNNA3 is a tumor suppressor in hepatocellular carcinomas and is inhibited by miR-425.Oncotarget · 2016Article
- Review
- Synaptopodin couples epithelial contractility to α-actinin-4-dependent junction maturation.The Journal of cell biology · 2015Article
- Nanoscale protein domain motion and long-range allostery in signaling proteins- a view from neutron spin echo sprectroscopy.Biophysical reviews · 2015Article
- Mapping the dynamics of force transduction at cell-cell junctions of epithelial clusters.eLife · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
The progression of several human cancers correlates with the loss of cytoplasmic protein alpha-catenin from E-cadherin-rich intercellular junctions and loss of adhesion. However, the potential role of alpha-catenin in directly modulating the adhesive function of individual E-cadherin molecules in human cancer is unknown. Here we use single-molecule force spectroscopy to probe the tensile strength, unstressed bond lifetime, and interaction energy between E-cadherins expressed on the surface of live human parental breast cancer cells lacking alpha-catenin and these cells where alpha-catenin is re-expressed. We find that the tensile strength and the lifetime of single E-cadherin/E-cadherin bonds between parental cells are significantly lower over a wide range of loading rates. Statistical analysis of the force displacement spectra reveals that single cadherin bonds between cancer cells feature an exceedingly low energy barrier against tensile forces and low molecular stiffness. Disassembly of filamentous actin using latrunculin B has no significant effect on the strength of single intercellular E-cadherin bonds. The absence of alpha-catenin causes a dominant negative effect on both global cell-cell adhesion and single E-cadherin bond strength. These results suggest that the loss of alpha-catenin alone drastically reduces the adhesive force between individual cadherin pairs on adjoining cells, explain the global loss of cell adhesion in human breast cancer cells, and show that the forced expression of alpha-catenin in cancer cells can restore both higher intercellular avidity and intercellular E-cadherin bond strength.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.