ArticleJournal of virology2009
Cryo-electron microscopy structure of adenovirus type 2 temperature-sensitive mutant 1 reveals insight into the cell entry defect.
Article in Journal of virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Structure of a Cell Entry Defective Human Adenovirus Provides Insights into Precursor Proteins and Capsid Maturation.Journal of molecular biology · 2022Article
- Dynamic competition for hexon binding between core protein VII and lytic protein VI promotes adenovirus maturation and entry.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Adeno-associated viral vector-mediated immune responses: Understanding barriers to gene delivery.Pharmacology & therapeutics · 2020Review
- Atomic Structures of Minor Proteins VI and VII in Human Adenovirus.Journal of virology · 2017Article
- Role of Condensing Particles in Polymer Confinement: A Model for Virus-Packed "Minichromosomes".Biophysical journal · 2017Article
- The adenovirus major core protein VII is dispensable for virion assembly but is essential for lytic infection.PLoS pathogens · 2017Article
- Cryo-EM structure of human adenovirus D26 reveals the conservation of structural organization among human adenoviruses.Science advances · 2017Article
- Insights into Adenovirus Uncoating from Interactions with Integrins and Mediators of Host Immunity.Viruses · 2016Review
- Structures of Adenovirus Incomplete Particles Clarify Capsid Architecture and Show Maturation Changes of Packaging Protein L1 52/55k.Journal of virology · 2015Article
- Review
- The amphipathic helix of adenovirus capsid protein VI contributes to penton release and postentry sorting.Journal of virology · 2015Article
- Review
- Coat as a dagger: the use of capsid proteins to perforate membranes during non-enveloped DNA viruses trafficking.Viruses · 2014Review
- The cleaved N-terminus of pVI binds peripentonal hexons in mature adenovirus.Journal of molecular biology · 2014Article
- Regulation of a viral proteinase by a peptide and DNA in one-dimensional space: I. binding to DNA AND to hexon of the precursor to protein VI, pVI, of human adenovirus.The Journal of biological chemistry · 2013Article
- Monitoring dynamics of human adenovirus disassembly induced by mechanical fatigue.Scientific reports · 2013Article
- Reduced infectivity of adenovirus type 5 particles and degradation of entering viral genomes associated with incomplete processing of the preterminal protein.Journal of virology · 2012Article
- The role of capsid maturation on adenovirus priming for sequential uncoating.The Journal of biological chemistry · 2012Article
- Disulfide-bond formation by a single cysteine mutation in adenovirus protein VI impairs capsid release and membrane lysis.Virology · 2012Article
- Latest insights on adenovirus structure and assembly.Viruses · 2012Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The structure of the adenovirus type 2 temperature-sensitive mutant 1 (Ad2ts1) was determined to a resolution of 10 A by cryo-electron microscopy single-particle reconstruction. Ad2ts1 was prepared at a nonpermissive temperature and contains the precursor forms of the capsid proteins IIIa, VI, and VIII; the core proteins VII, X (mu), and terminal protein (TP); and the L1-52K protein. Cell entry studies have shown that although Ad2ts1 can bind the coxsackievirus and Ad receptor and undergo internalization via alphav integrins, this mutant does not escape from the early endosome and is targeted for degradation. Comparison of the Ad2ts1 structure to that of mature Ad indicates that Ad2ts1 has a different core architecture. The Ad2ts1 core is closely associated with the icosahedral capsid, a connection which may be mediated by preproteins IIIa and VI. Density within hexon cavities is assigned to preprotein VI, and membrane disruption assays show that hexon shields the lytic activity of both the mature and precursor forms of protein VI. The internal surface of the penton base in Ad2ts1 appears to be anchored to the core by interactions with preprotein IIIa. Our structural analyses suggest that these connections to the core inhibit the release of the vertex proteins and lead to the cell entry defect of Ad2ts1.
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