ArticleBMC microbiology2009
A plant MinD homologue rescues Escherichia coli HL1 mutant (DeltaMinDE) in the absence of MinE.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
7 citing papers in PubMed, 13 citations in OpenAlex.
- Septum site placement inMicrobiology (Reading, England) · 2023Article
- Fluorescent Labeling and Confocal Microcopy of Plastids and Stromules.Methods in molecular biology (Clifton, N.J.) · 2021Article
- The chloroplast division protein ARC6 acts to inhibit disassembly of GDP-bound FtsZ2.The Journal of biological chemistry · 2018Article
- Self-organization principles of intracellular pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Geometry-induced protein pattern formation.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Differences in MinC/MinD sensitivity between polar and internal Z rings in Escherichia coli.Journal of bacteriology · 2011Article
- Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa.BMC genomics · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIn E. coli, the Min operon (MinCDE) plays a key role in determining the site of cell division. MinE oscillates from the middle to one pole or another to drive the MinCD complex to the end of the cell. The MinCD complex prevents FtsZ ring formation and the subsequent cell division at cell ends. In Arabidopsis thaliana, a homologue of MinD has been shown to be involved in the positioning of chloroplast division site.
resultsTo learn whether the MinD homologue in plants is functional in bacteria, AtMinD was expressed in E. coli. Surprisingly, AtMinD can rescue the minicell phenotype of E. coli HL1 mutant (DeltaMinDE) in the absence of EcMinE. This rescue requires EcMinC. AtMinD was localized to puncta at the poles of E. coli cells and puncta in chloroplasts without oscillation. AtMinD expressed in the HL1 mutant can cause a punctate localization pattern of GFP-EcMinC at cell ends. Yeast two hybrid and BiFC analysis showed that AtMinD can interact with EcMinC.
conclusionSimilar to the MinD in Bacillus subtilis, AtMinD is localized to the polar region in E. coli and interacts with EcMinC to confine EcFtsZ polymerization and cell division at the midpoint of the cell.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.