ArticlePlant cell reports2009
Piercing and vacuum infiltration of the mature embryo: a simplified method for Agrobacterium-mediated transformation of indica rice.
Article in Plant cell reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
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- Crop genome editing through tissue-culture-independent transformation methods.Frontiers in genome editing · 2024Review
- A novel approach: enhancing marigold (Frontiers in plant science · 2024Article
- Stable In-Planta Transformation System For Egyptian Sesame (GM crops & food · 2023Article
- Agrobacterium-mediated in planta transformation of cut coleoptile: a new, simplified, and tissue culture-independent method to deliver the CRISPR/Cas9 system in rice.Molecular biology reports · 2023Article
- The protein phosphatase PC1 dephosphorylates and deactivates CatC to negatively regulate H2O2 homeostasis and salt tolerance in rice.The Plant cell · 2023Article
- CRISPR/Cas9-Mediated Cytosine Base Editing Using an Improved Transformation Procedure in Melon (International journal of molecular sciences · 2023Article
- Establishment of Agrobacterium-mediated transformation system to Juglans sigillata Dode 'Qianhe-7'.Transgenic research · 2023Article
- Optimisation of a rapid and efficient transformation protocol for fungal blast-susceptible indica rice cultivars HR-12 and CO-39.Plant biotechnology (Tokyo, Japan) · 2021Article
- A Novel Embryo Phenotype Associated With Interspecific Hybrid Weakness in Rice Is Controlled by the MADS-Domain Transcription FactorFrontiers in plant science · 2021Article
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- RcPAL, a key gene in lignin biosynthesis in Ricinus communis L.BMC plant biology · 2019Article
- Cloning, transformation and expression of cell cycle-associated protein kinaseJournal, genetic engineering & biotechnology · 2018Article
- The Receptor-Like Cytoplasmic Kinase STRK1 Phosphorylates and Activates CatC, Thereby Regulating HThe Plant cell · 2018Article
- Opportunities for Innovation in Genetic Transformation of Forest Trees.Frontiers in plant science · 2018Review
- The Rice AAA-ATPase OsFIGNL1 Is Essential for Male Meiosis.Frontiers in plant science · 2017Article
- In planta Transformed Cumin (Cuminum cyminum L.) Plants, Overexpressing the SbNHX1 Gene Showed Enhanced Salt Endurance.PloS one · 2016Article
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Authors and funding
9 authors.
Funding
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Abstract
Traditional transformation methods are complex and time consuming. It is generally difficult to transform indica rice varieties using traditional transformation methods due to their poor regeneration. In this contribution, a simple method was developed for the transformation of indica rice. In this method, the mature embryos of soaked seeds were pierced by a needle, and then soaked in the Agrobacterium inoculum under vacuum infiltration. The inoculated seeds germinated and grew to maturation (T (0)) under nonsterile conditions. The herbicide or antibiotic analysis and molecular analysis were conducted on T (0) plants. The results showed that although the efficiency of transformation was about 6.0%, it was easier to transform indica rice using the proposed method, and the transformation process was significantly shortened. The success of transformation was further confirmed by the genetic and molecular analyses of T (1) transformants.
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