ArticleThe Plant cell2009
Local differentiation of sugar donor specificity of flavonoid glycosyltransferase in Lamiales.
Article in The Plant cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
57 citing papers in PubMed, 135 citations in OpenAlex.
- Advances in UDP-Glycosyltransferases from Medicinal Plants: Discovery, Catalytic Mechanism, Engineering and Biosynthetic Application.Metabolites · 2026Review
- Dynamic diversification of lignan metabolism in sesame via coordinated oxygenation and glucosylation across germination.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- Boosting the catalytic efficiency of UGT51 for efficient production of rare ginsenoside Rh2.Folia microbiologica · 2025Article
- Transcriptomic and Metabolomic Insights into Key Genes Involved in Kinsenoside Biosynthesis inPlants (Basel, Switzerland) · 2025Article
- The multi-pathway treatment of flavonoids as natural compounds in neurological diseases: achievements, limitations, and prospects.Frontiers in neuroscience · 2025Review
- Flavonoids: a natural remedy in the prevention and management of diverse diseases.Frontiers in medicine · 2025Review
- Metabolic Engineering ofMolecules (Basel, Switzerland) · 2024Review
- Article
- The sugar donor specificity of plant family 1 glycosyltransferases.Frontiers in bioengineering and biotechnology · 2024Review
- Multi-omics landscape to decrypt the distinct flavonoid biosynthesis ofHorticulture research · 2024Article
- Biochemical Characterization of Parsley Glycosyltransferases Involved in the Biosynthesis of a Flavonoid Glycoside, Apiin.International journal of molecular sciences · 2023Article
- The apiosyltransferase celery UGT94AX1 catalyzes the biosynthesis of the flavone glycoside apiin.Plant physiology · 2023Article
- Applications of protein engineering in the microbial synthesis of plant triterpenoids.Synthetic and systems biotechnology · 2023Review
- The chemical profiling ofFrontiers in plant science · 2023Article
- Systematic analysis of the UDP-glucosyltransferase family: discovery of a member involved in rutin biosynthesis inFrontiers in plant science · 2023Article
- Silencing ofPlants (Basel, Switzerland) · 2022Article
- UGT72, a Major Glycosyltransferase Family for Flavonoid and Monolignol Homeostasis in Plants.Biology · 2022Review
- Characterization of UDP-glycosyltransferase family members reveals how major flavonoid glycoside accumulates in the roots of Scutellaria baicalensis.BMC genomics · 2022Article
- Parallel evolution of UbiA superfamily proteins into aromaticProceedings of the National Academy of Sciences of the United States of America · 2021Article
- UGT84F9 is the major flavonoid UDP-glucuronosyltransferase in Medicago truncatula.Plant physiology · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Flavonoids are most commonly conjugated with various sugar moieties by UDP-sugar:glycosyltransferases (UGTs) in a lineage-specific manner. Generally, the phylogenetics and regiospecificity of flavonoid UGTs are correlated, indicating that the regiospecificity of UGT differentiated prior to speciation. By contrast, it is unclear how the sugar donor specificity of UGTs evolved. Here, we report the biochemical, homology-modeled, and phylogenetic characterization of flavonoid 7-O-glucuronosyltransferases (F7GAT), which is responsible for producing specialized metabolites in Lamiales plants. All of the Lamiales F7GATs were found to be members of the UGT88-related cluster and specifically used UDP-glucuronic acid (UDPGA). We identified an Arg residue that is specifically conserved in the PSPG box in the Lamiales F7GATs. Substitution of this Arg with Trp was sufficient to convert the sugar donor specificity of the Lamiales F7GATs from UDPGA to UDP-glucose. Homology modeling of the Lamiales F7GAT suggested that the Arg residue plays a critical role in the specific recognition of anionic carboxylate of the glucuronic acid moiety of UDPGA with its cationic guanidinium moiety. These results support the hypothesis that differentiation of sugar donor specificity of UGTs occurred locally, in specific plant lineages, after establishment of general regiospecificity for the sugar acceptor. Thus, the plasticity of sugar donor specificity explains, in part, the extraordinary structural diversification of phytochemicals.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.