ArticleJournal of immunology (Baltimore, Md. : 1950)2009
In situ IL-12/23p40 production during mycobacterial infection is sustained by CD11bhigh dendritic cells localized in tissue sites distinct from those harboring bacilli.
Article in Journal of immunology (Baltimore, Md. : 1950), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Glucose metabolism and its role in the maturation and migration of human CD1cFrontiers in cellular and infection microbiology · 2023Article
- IL-12p40 is essential but not sufficient for Francisella tularensis LVS clearance in chronically infected mice.PloS one · 2023Article
- Cyclooxygenase-Derived Prostaglandin EJournal of immunology (Baltimore, Md. : 1950) · 2022Article
- Vaccinia Virus Infection Inhibits Skin Dendritic Cell Migration to the Draining Lymph Node.Journal of immunology (Baltimore, Md. : 1950) · 2021Article
- STAT3 expression by myeloid cells is detrimental for the T- cell-mediated control of infection with Mycobacterium tuberculosis.PLoS pathogens · 2018Article
- LangerinFrontiers in immunology · 2018Article
- Immunogenicity is preferentially induced in sparse dendritic cell cultures.Scientific reports · 2017Article
- Mycobacteria induce TPL-2 mediated IL-10 in IL-4-generated alternatively activated macrophages.PloS one · 2017Article
- Outrunning the Red Queen: bystander activation as a means of outpacing innate immune subversion by intracellular pathogens.Cellular & molecular immunology · 2017Review
- Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Dental follicle mesenchymal stem cell administration ameliorates muscle weakness in MuSK-immunized mice.Journal of neuroinflammation · 2015Article
- BCG Skin Infection Triggers IL-1R-MyD88-Dependent Migration of EpCAMlow CD11bhigh Skin Dendritic cells to Draining Lymph Node During CD4+ T-Cell Priming.PLoS pathogens · 2015Article
- IL12Rβ1: the cytokine receptor that we used to know.Cytokine · 2015Review
- Beyond macrophages: the diversity of mononuclear cells in tuberculosis.Immunological reviews · 2014Review
- Cell-to-cell transfer of M. tuberculosis antigens optimizes CD4 T cell priming.Cell host & microbe · 2014Article
- The glycosylated Rv1860 protein of Mycobacterium tuberculosis inhibits dendritic cell mediated TH1 and TH17 polarization of T cells and abrogates protective immunity conferred by BCG.PLoS pathogens · 2014Article
- IL12B expression is sustained by a heterogenous population of myeloid lineages during tuberculosis.Tuberculosis (Edinburgh, Scotland) · 2013Article
- Article
- TLR-2 independent recognition of Mycobacterium tuberculosis by CD11c+ pulmonary cells from old mice.Mechanisms of ageing and development · 2010Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 3 countries.
Funding
Abstract
Although IL-12/23p40 is known to play a major role in host resistance to Mycobacterium spp, the cellular source, tissue localization, and regulation of p40 production during mycobacterial infection in vivo has been unclear. In this study, we used IL-12/23p40eYFP (yet40) reporter mice to track expression of the cytokine following Mycobacterium bovis bacillus Calmette-Guérin (BCG) infection. We found that in spleens of these mice, p40 production is initiated by a transient burst from CD11b(low)CD11c(+) dendritic cells (DC) which are later replaced at the onset of granuloma formation by CD11b(high)CD11c(+) DC as the major source of the cytokine. The latter subset was also found to be the key producer of DC-derived p40 in nonlymphoid tissue and in both spleen and liver optimal production of the cytokine was regulated by endogenous TNF-alpha. Although BCG and p40-expressing DC were both observed in splenic white pulp, p40(+) DC rarely colocalized with bacilli. Indeed, in vitro flow cytometry and confocal microscopy indicated that the presence of intracellular bacteria is not required for p40 production by DC and Transwell experiments confirmed that soluble mycobacterial components are sufficient for inducing cytokine expression by these cells. Moreover, when stimulated with LPS, DC directly infected with BCG showed impaired IL-12p40 production in vitro. Together, our findings establish CD11b(high) DC as a major source of IL-12/23p40 during mycobacterial infection in situ and implicate both soluble mycobacterial products and TNF-alpha in stimulating sustained production of p40 by these cells.
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