ArticleBMC microbiology2009
Molecular beacon-based real-time PCR detection of primary isolates of Salmonella Typhimurium and Salmonella Enteritidis in environmental and clinical samples.
Article in BMC microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Effect of zinc on growth performance, gut morphometry, and cecal microbial community in broilers challenged with Salmonella enterica serovar typhimurium.Journal of microbiology (Seoul, Korea) · 2014Trial
- Evaluation of mdh, dld, tcfA, and folE gene markers for detection of enteric fever using real-time PCR.Scientific reports · 2026Article
- One-tube detection ofMicrobiology spectrum · 2024Article
- Article
- Prevalence of Salmonella Typhimurium and Salmonella Enteritidis isolated from poultry meat: virulence and antimicrobial-resistant genes.BMC microbiology · 2023Article
- Progress in Fluorescence Biosensing and Food Safety towards Point-of-Detection (PoD) System.Biosensors · 2023Review
- Assessment and Comparison of Molecular Subtyping and Characterization Methods forFrontiers in microbiology · 2019Review
- Article
- Real-Time PCR Method for Detection of Salmonella spp. in Environmental Samples.Applied and environmental microbiology · 2017Article
- An application of competitive reporter monitored amplification (CMA) for rapid detection of single nucleotide polymorphisms (SNPs).PloS one · 2017Article
- Evaluation of a multiplex PCR method to serotypeMethodsX · 2017Article
- Rapid bovine and caprine species identification in ruminant feeds by duplex real-time PCR melting curve analysis using EvaGreen fluorescence dye.Molecular biotechnology · 2014Article
- Isolation and characterization of Salmonella enterica in day-old ducklings in Egypt.Pathogens and global health · 2014Article
- Using metagenomic analyses to estimate the consequences of enrichment bias for pathogen detection.BMC research notes · 2012Article
- Molecular beacons in diagnostics.F1000 medicine reports · 2012Article
- Development of a molecular-beacon-based multi-allelic real-time RT-PCR assay for the detection of human coronavirus causing severe acute respiratory syndrome (SARS-CoV): a general methodology for detecting rapidly mutating viruses.Archives of virology · 2011Article
- Molecular typing of Salmonella spp isolated from food handlers and animals in Nigeria.International journal of molecular epidemiology and genetics · 2011Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA fast and simple two-step multiplex real-time PCR assay has been developed to replace the traditional, laborious Salmonella serotyping procedure. Molecular beacons were incorporated into the assay as probes for target DNA. Target sequences were regions of the invA, prot6E and fliC genes specific for Salmonella spp. Salmonella Enteritidis and Salmonella Typhimurium, respectively, the two most clinically relevant serotypes. An internal amplification positive control was included in the experiment to ensure the optimal functioning of the PCR and detect possible PCR inhibition. Three sets of primers were used for the amplification of the target sequences. The results were compared to those of the Kauffmann-White antigenic classification scheme.
resultsThe assay was 100% sensitive and specific, correctly identifying all 44 Salmonella strains, all 21 samples of S. Enteritidis and all 17 samples of S. Typhimurium tested in this work. Therefore, the entire experiment had specificity and sensitivity of 100%. The detection limit was down to 10 copies of DNA target per 25 microl reaction.
conclusionThe assay can amplify and analyse a large number of samples in approximately 8 hours, compared to the 4 to 5 days conventional identification takes, and is thus considered a very promising method for detecting the two major serotypes of Salmonella quickly and accurately from clinical and environmental samples.
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