ArticleJournal of cellular and molecular medicine2010
Automatic retrieval of single microchimeric cells and verification of identity by on-chip multiplex PCR.
Article in Journal of cellular and molecular medicine, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Identifying Key Questions and Challenges in Microchimerism Biology.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- The Microchimerism Literature Atlas.Bioinformatics and biology insights · 2025Article
- Unravelling the biological secrets of microchimerism by single-cell analysis.Briefings in functional genomics · 2018Article
- Target Cell Pre-enrichment and Whole Genome Amplification for Single Cell Downstream Characterization.Journal of visualized experiments : JoVE · 2018Article
- Beyond laser microdissection technology: follow the yellow brick road for cancer research.American journal of cancer research · 2014Review
- Naturally acquired microchimerism: implications for transplantation outcome and novel methodologies for detection.Chimerism · 2014Review
- The otherness of self: microchimerism in health and disease.Trends in immunology · 2012Review
- Combined molecular genetic and cytogenetic analysis from single cells after isothermal whole-genome amplification.Clinical chemistry · 2011Article
- Article
- Profile of a serial killer: cellular and molecular approaches to study individual cytotoxic T-cells following therapeutic vaccination.Journal of biomedicine & biotechnology · 2011Review
- Article
Corrections and comments
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The analysis of rare cells is not an easy task. This is especially true when cells representing a fetal microchimerism are to be utilized for the purpose of non-invasive prenatal diagnosis because it is both imperative and difficult to avoid contaminating the minority of fetal cells with maternal ones. Under these conditions, even highly specific biochemical markers are not perfectly reliable. We have developed a method to verify the genomic identity of rare cells that combines automatic screening for enriched target cells (based on immunofluorescence labelling) with isolation of single candidate microchimeric cells (by laser microdissection and subsequent laser catapulting) and low-volume on-chip multiplex PCR for DNA fingerprint analysis. The power of the method was tested using samples containing mixed cells of related and non-related individuals. Single-cell DNA fingerprinting was successful in 74% of the cells analysed (55/74), with a PCR efficiency of 59.2% (860/1452) for heterozygous loci. The identification of cells by means of DNA profiling was achieved in 100% (12/12) of non-related cells in artificial mixtures and in 86% (37/43) of cells sharing a haploid set of chromosomes and was performed on cells enriched from blood and cells isolated from tissue. We suggest DNA profiling as a standard for the identification of microchimerism on a single-cell basis.
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