ArticleThe Plant journal : for cell and molecular biology2009
ARS5 is a component of the 26S proteasome complex, and negatively regulates thiol biosynthesis and arsenic tolerance in Arabidopsis.
Article in The Plant journal : for cell and molecular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 71 citations in OpenAlex.
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- Plants' molecular behavior to heavy metals: from criticality to toxicity.Frontiers in plant science · 2024Review
- VOZ1 and VOZ2 transcription factors regulate arsenic tolerance and distribution in rice and Arabidopsis.Frontiers in plant science · 2023Article
- Conuping BSA-Seq and RNA-Seq Reveal the Molecular Pathway and Genes Associated with the Plant Height of Foxtail Millet (International journal of molecular sciences · 2022Article
- The transcription factor MYB40 is a central regulator in arsenic resistance inPlant communications · 2021Article
- Differences in stress defence mechanisms in germinating seeds of Pinus sylvestris exposed to various lead chemical forms.PloS one · 2020Article
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- Physiological and quantitative proteomic analyses unraveling potassium deficiency stress response in alligator weed (Alternanthera philoxeroides L.) root.Plant molecular biology · 2018Article
- Target proteins reprogrammed by As and As + Si treatments in Solanum lycopersicum L. fruit.BMC plant biology · 2017Article
- Abiotic Stress Phenotypes Are Associated with Conserved Genes Derived from Transposable Elements.Frontiers in plant science · 2017Article
- Cytokinin Determines Thiol-Mediated Arsenic Tolerance and Accumulation.Plant physiology · 2016Article
- Arabidopsis PROTEASOME REGULATOR1 is required for auxin-mediated suppression of proteasome activity and regulates auxin signalling.Nature communications · 2016Article
- Investigating the roles of ascorbate-glutathione cycle and thiol metabolism in arsenate tolerance in ridged Luffa seedlings.Protoplasma · 2015Article
- Regulation of Cadmium-Induced Proteomic and Metabolic Changes by 5-Aminolevulinic Acid in Leaves of Brassica napus L.PloS one · 2015Article
- Construction of a Modular Arsenic-Resistance Operon in E. coli and the Production of Arsenic Nanoparticles.Frontiers in bioengineering and biotechnology · 2015Article
- Article
- WRKY6 transcription factor restricts arsenate uptake and transposon activation in Arabidopsis.The Plant cell · 2013Article
- Reversion of the Arabidopsis rpn12a-1 exon-trap mutation by an intragenic suppressor that weakens the chimeric 5' splice site.F1000Research · 2013Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A forward-genetic screen in Arabidopsis led to the isolation of several arsenic tolerance mutants. ars5 was the strongest arsenate- and arsenite-resistant mutant identified in this genetic screen. Here, we report the characterization and cloning of the ars5 mutant gene. ars5 is shown to exhibit an increased accumulation of arsenic and thiol compounds during arsenic stress. Rough mapping together with microarray-based expression mapping identified the ars5 mutation in the alpha subunit F (PAF1) of the 26S proteasome complex. Characterization of an independent paf1 T-DNA insertion allele and complementation by PAF1 confirmed that paf1 mutation is responsible for the enhanced thiol accumulation and arsenic tolerance phenotypes. Arsenic tolerance was not observed in a knock-out mutant of the highly homologous PAF2 gene. However, genetic complementation of ars5 by the overexpression of PAF2 suggests that the PAF2 protein is functionally equivalent to PAF1 when expressed at high levels. No detectible difference was observed in total ubiquitinylated protein profiles between ars5 and wild-type (WT) Arabidopsis, suggesting that the arsenic tolerance observed in ars5 is not derived from a general impairment in proteasome-mediated protein degradation. Quantitative RT-PCR showed that arsenic induces the enhanced transcriptional activation of several key genes that function in glutathione and phytochelatin biosynthesis in the WT, and this arsenic induction of gene expression is more dramatic in ars5. The enhanced transcriptional response to arsenic and the increased accumulation of thiol compounds in ars5, compared with WT, suggest the presence of a positive regulation pathway for thiol biosynthesis that is enhanced in the ars5 background.
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