ArticleInfluenza and other respiratory viruses2007
Oligonucleotide microchip for subtyping of influenza A virus.
Article in Influenza and other respiratory viruses, 2007. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Development of DNA Microarray for Parallel Detection of Community-Acquired Pneumonia Bacterial Pathogens.Sovremennye tekhnologii v meditsine · 2024Article
- Identification of genetic determinants of influenza A virus resistance to adamantanes and neuraminidase inhibitors using biological microarray.Doklady. Biochemistry and biophysics · 2015Article
- Comparative evaluation of effectiveness of IAVchip DNA microarray in influenza A diagnosis.TheScientificWorldJournal · 2014Article
- Design of a set of probes with high potential for influenza virus epidemiological surveillance.Bioinformation · 2013Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundInfluenza A viruses are classified into subtypes depending on the antigenic properties of their two outer glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Sixteen subtypes of HA and nine of NA are known. Lately, the circulation of some subtypes (H7N7, H5N1) has been closely watched because of the epidemiological threat they present.
objectivesThis study assesses the potential of using gel-based microchip technology for fast and sensitive molecular subtyping of the influenza A virus.
methodsThe method employs a microchip of 3D gel-based elements containing immobilized probes. Segments of the HA and NA genes are amplified using multiplex RT-PCR and then hybridized with the microchip.
resultsThe developed microchip was validated using a panel of 21 known reference strains of influenza virus. Selected strains represented different HA and NA subtypes derived from avian, swine and human hosts. The whole procedure takes 10 hours and enables one to identify 15 subtypes of HA and two subtypes of NA. Forty-one clinical samples isolated during the poultry fall in Novosibirsk (Russia, 2005) were successfully identified using the proposed technique. The sensitivity and specificity of the method were 76% and 100%, respectively, compared with the 'gold standard' techniques (virus isolation with following characterization by immunoassay).
conclusionsWe conclude that the method of subtyping using gel-based microchips is a promising approach for fast detection and identification of influenza A, which may greatly improve its monitoring.
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