ArticleJournal of molecular recognition : JMR
Combining atomic force and fluorescence microscopy for analysis of quantum-dot labeled protein-DNA complexes.
Article in Journal of molecular recognition : JMR. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Single-molecule optical mapping of the distribution of DNA phosphorothioate epigenetics.Nucleic acids research · 2021Article
- Studying protein-DNA interactions using atomic force microscopy.Seminars in cell & developmental biology · 2018Review
- Direct optical mapping of transcription factor binding sites on field-stretched λ-DNA in nanofluidic devices.Nucleic acids research · 2014Article
- Toward single-molecule optical mapping of the epigenome.ACS nano · 2014Article
- Investigating bioconjugation by atomic force microscopy.Journal of nanobiotechnology · 2013Review
- Combined optical and topographic imaging reveals different arrangements of human RAD54 with presynaptic and postsynaptic RAD51-DNA filaments.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Enzymatically incorporated genomic tags for optical mapping of DNA-binding proteins.Angewandte Chemie (International ed. in English) · 2012Article
- High accuracy FIONA-AFM hybrid imaging.Ultramicroscopy · 2011Article
- Molecular recognition of DNA-protein complexes: a straightforward method combining scanning force and fluorescence microscopy.Ultramicroscopy · 2010Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Atomic force microscopy (AFM) and fluorescence microscopy are widely used for the study of protein-DNA interactions. While AFM excels in its ability to elucidate structural detail and spatial arrangement, it lacks the ability to distinguish between similarly sized objects in a complex system. This information is readily accessible to optical imaging techniques via site-specific fluorescent labels, which enable the direct detection and identification of multiple components simultaneously. Here, we show how the utilization of semiconductor quantum dots (QDs), serving as contrast agents for both AFM topography and fluorescence imaging, facilitates the combination of both imaging techniques, and with the addition of a flow based DNA extension method for sample deposition, results in a powerful tool for the study of protein-DNA complexes. We demonstrate the inherent advantages of this novel combination of techniques by imaging individual RNA polymerases (RNAP) on T7 genomic DNA.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.