ArticleCurrent genomics2008
Application of TILLING and EcoTILLING as Reverse Genetic Approaches to Elucidate the Function of Genes in Plants and Animals.
Article in Current genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 118 citations in OpenAlex.
- Perennials as Future Grain Crops: Opportunities and Challenges.Frontiers in plant science · 2022Pooled it
- Arabidopsis as a model for translational research.The Plant cell · 2025Review
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- Beyond the genome: the role of functional markers in contemporary plant breeding.Frontiers in plant science · 2025Review
- Allele mining of eukaryotic translation initiation factor genes in Prunus for the identification of new sources of resistance to sharka.Scientific reports · 2023Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- PANOMICS meets germplasm.Plant biotechnology journal · 2020Review
- In vitro androgenesis: spontaneous vs. artificial genome doubling and characterization of regenerants.Plant cell reports · 2020Review
- Deployment of Genetic and Genomic Tools Toward Gaining a Better Understanding of Rice-Frontiers in plant science · 2020Review
- Mutagenesis in Rice: The Basis for Breeding a New Super Plant.Frontiers in plant science · 2019Review
- Functional genomic approaches to improve crop plant heat stress tolerance.F1000Research · 2019Review
- EcoTILLING by sequencing reveals polymorphisms in genes encoding starch synthases that are associated with low glycemic response in rice.BMC plant biology · 2017Article
- Compact tomato seedlings and plants upon overexpression of a tomato chromatin remodelling ATPase gene.Plant biotechnology journal · 2016Article
- EcoTILLING-Based Association Mapping Efficiently Delineates Functionally Relevant Natural Allelic Variants of Candidate Genes Governing Agronomic Traits in Chickpea.Frontiers in plant science · 2016Article
- Breeding Vegetables with Increased Content in Bioactive Phenolic Acids.Molecules (Basel, Switzerland) · 2015Review
- The potential of transcription factor-based genetic engineering in improving crop tolerance to drought.Omics : a journal of integrative biology · 2014Review
- SMART--Sunflower Mutant population And Reverse genetic Tool for crop improvement.BMC plant biology · 2013Article
- Induction, rapid fixation and retention of mutations in vegetatively propagated banana.Plant biotechnology journal · 2012Article
- Integrated genomics, physiology and breeding approaches for improving drought tolerance in crops.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Review
- Application of genomic tools in plant breeding.Current genomics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the fairly recent advent of inexpensive, rapid sequencing technologies that continue to improve sequencing efficiency and accuracy, many species of animals, plants, and microbes have annotated genomic information publicly available. The focus on genomics has thus been shifting from the collection of whole sequenced genomes to the study of functional genomics. Reverse genetic approaches have been used for many years to advance from sequence data to the resulting phenotype in an effort to deduce the function of a gene in the species of interest. Many of the currently used approaches (RNAi, gene knockout, site-directed mutagenesis, transposon tagging) rely on the creation of transgenic material, the development of which is not always feasible for many plant or animal species. TILLING is a non-transgenic reverse genetics approach that is applicable to all animal and plant species which can be mutagenized, regardless of its mating / pollinating system, ploidy level, or genome size. This approach requires prior DNA sequence information and takes advantage of a mismatch endonuclease to locate and detect induced mutations. Ultimately, it can provide an allelic series of silent, missense, nonsense, and splice site mutations to examine the effect of various mutations in a gene. TILLING has proven to be a practical, efficient, and an effective approach for functional genomic studies in numerous plant and animal species. EcoTILLING, which is a variant of TILLING, examines natural genetic variation in populations and has been successfully utilized in animals and plants to discover SNPs including rare ones. In this review, TILLING and EcoTILLING techniques, beneficial applications and limitations from plant and animal studies are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.