ArticleProceedings of the National Academy of Sciences of the United States of America2009
Light-transduction in melanopsin-expressing photoreceptors of Amphioxus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 43 citations in OpenAlex.
- A 3D amphioxus brain atlas illuminates the blueprint of the ancestral chordate brain and highlights unique vertebrate innovations.Science advances · 2026Article
- Gene networks and the evolution of olfactory organs, eyes, hair cells and motoneurons: a view encompassing lancelets, tunicates and vertebrates.Frontiers in cell and developmental biology · 2024Review
- Deep Diversity: Extensive Variation in the Components of Complex Visual Systems across Animals.Cells · 2022Review
- Vision and retina evolution: How to develop a retina.IBRO neuroscience reports · 2022Article
- PACAP/GCGa Is an Important Modulator of the Amphioxus CNS-Hatschek's Pit Axis, the Homolog of the Vertebrate Hypothalamic-Pituitary Axis in the Basal Chordates.Frontiers in endocrinology · 2022Article
- Melanopsin phototransduction: beyond canonical cascades.The Journal of experimental biology · 2021Review
- Slowly seeing the light: an integrative review on ecological light pollution as a potential threat for mollusks.Environmental science and pollution research international · 2021Review
- The visual pigment xenopsin is widespread in protostome eyes and impacts the view on eye evolution.eLife · 2020Article
- Melanopsin and the Intrinsically Photosensitive Retinal Ganglion Cells: Biophysics to Behavior.Neuron · 2019Review
- Co-expression of xenopsin and rhabdomeric opsin in photoreceptors bearing microvilli and cilia.eLife · 2017Article
- The evolution of rod photoreceptors.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2017Review
- Calcium activates the light-dependent conductance in melanopsin-expressing photoreceptors of amphioxus.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Progenitors of the protochordate ocellus as an evolutionary origin of the neural crest.EvoDevo · 2013Article
- Dissecting the determinants of light sensitivity in amphioxus microvillar photoreceptors: possible evolutionary implications for melanopsin signaling.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Melanopsin and mechanisms of non-visual ocular photoreception.The Journal of biological chemistry · 2012Review
- Melanopsin-expressing amphioxus photoreceptors transduce light via a phospholipase C signaling cascade.PloS one · 2012Article
- The light-sensitive conductance of melanopsin-expressing Joseph and Hesse cells in amphioxus.The Journal of general physiology · 2012Article
- Melanopsin signalling in mammalian iris and retina.Nature · 2011Article
- Unique system of photoreceptors in sea urchin tube feet.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Intrinsically photosensitive retinal ganglion cells.Physiological reviews · 2010Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spatial vision in different organisms is mediated by 2 classes of photoreceptors: microvillar and ciliary. Recently, additional photosensitive cells implicated in nonvisual light-dependent functions have been identified in the mammalian retina. A previously undescribed photopigment, melanopsin, underlies these photoresponses, and it has been proposed that its transduction mechanisms may be akin to the lipid-signaling scheme of invertebrate microvillar receptors, rather than the cyclic-nucleotide cascade of vertebrates. Melanopsin has an ancient origin in deuterostomia, and expresses in 2 morphologically distinct classes of cells in the neural tube of Amphioxus, the most basal extant chordate: pigmented ocelli, and Joseph cells. However, to our knowledge, their physiology and alleged photosensitivity had never been investigated. We dissociated both types of cells, and conclusively demonstrated by patch-electrode recoding that they are primary photoreceptors; their receptor potential is depolarizing, accompanied by an increase in membrane conductance. The action spectrum peaks in the blue region, approximately 470 nm, similar to the absorption of melanopsin in vitro. The light-dependent conductance rectifies inwardly; Na and Ca are differentially implicated in the 2 cell types. Fluorescence Ca imaging reveals that photostimulation rapidly mobilizes calcium from internal stores. Intracellular 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate severely impairs the photoresponse, indicating that light-evoked Ca elevation is an important event in photoexcitation. These observations support the notion that the lineage of microvillar photoreceptors and its associated light-signaling pathway also evolved in the chordates. Thus, Joseph cells and pigmented ocelli of the Amphioxus may represent a link between ancestral rhabdomeric-like light sensors present in prebilaterians and the circadian photoreceptors of higher vertebrates.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.