ArticleMolecular and cellular biology2009
Chromatin structure is implicated in "late" elongation checkpoints on the U2 snRNA and beta-actin genes.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 41 citations in OpenAlex.
- CAPTURE of the Human U2 snRNA Genes Expands the Repertoire of Associated Factors.Biomolecules · 2022Article
- CTCF as a regulator of alternative splicing: new tricks for an old player.Nucleic acids research · 2021Review
- CDK9 keeps RNA polymerase II on track.Cellular and molecular life sciences : CMLS · 2021Review
- Alternative splicing of DSP1 enhances snRNA accumulation by promoting transcription termination and recycle of the processing complex.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Connections between 3' end processing and DNA damage response: Ten years later.Wiley interdisciplinary reviews. RNA · 2020Review
- Regulation of expression of human RNA polymerase II-transcribed snRNA genes.Open biology · 2017Review
- RNA polymerase II pausing as a context-dependent reader of the genome.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2016Review
- The point of no return: The poly(A)-associated elongation checkpoint.RNA biology · 2016Article
- Integrator: surprisingly diverse functions in gene expression.Trends in biochemical sciences · 2015Review
- CDK9 inhibitors define elongation checkpoints at both ends of RNA polymerase II-transcribed genes.Nature structural & molecular biology · 2015Article
- Epigenetic basis of opiate suppression of Bdnf gene expression in the ventral tegmental area.Nature neuroscience · 2015Article
- The Integrator complex controls the termination of transcription at diverse classes of gene targets.Cell research · 2015Article
- CTCF regulates NELF, DSIF and P-TEFb recruitment during transcription.Transcription · 2015Article
- Human snRNA genes use polyadenylation factors to promote efficient transcription termination.Nucleic acids research · 2014Article
- Article
- Drosha regulates gene expression independently of RNA cleavage function.Cell reports · 2013Article
- Functional analysis of the integrator subunit 12 identifies a microdomain that mediates activation of the Drosophila integrator complex.The Journal of biological chemistry · 2013Article
- CTCF regulates Kaposi's sarcoma-associated herpesvirus latency transcription by nucleosome displacement and RNA polymerase programming.Journal of virology · 2013Article
- SAYP and Brahma are important for 'repressive' and 'transient' Pol II pausing.Nucleic acids research · 2012Article
- Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The negative elongation factor NELF is a key component of an early elongation checkpoint generally located within 100 bp of the transcription start site of protein-coding genes. Negotiation of this checkpoint and conversion to productive elongation require phosphorylation of the carboxy-terminal domain of RNA polymerase II (pol II), NELF, and DRB sensitivity-inducing factor (DSIF) by positive transcription elongation factor b (P-TEFb). P-TEFb is dispensable for transcription of the noncoding U2 snRNA genes, suggesting that a NELF-dependent checkpoint is absent. However, we find that NELF at the end of the 800-bp U2 gene transcription unit and RNA interference-mediated knockdown of NELF causes a termination defect. NELF is also associated 800 bp downstream of the transcription start site of the beta-actin gene, where a "late" P-TEFb-dependent checkpoint occurs. Interestingly, both genes have an extended nucleosome-depleted region up to the NELF-dependent control point. In both cases, transcription through this region is P-TEFb independent, implicating chromatin in the formation of the terminator/checkpoint. Furthermore, CTCF colocalizes with NELF on the U2 and beta-actin genes, raising the possibility that it helps the positioning and/or function of the NELF-dependent control point on these genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.