ArticleMethods (San Diego, Calif.)2009
The Hermes transposon of Musca domestica and its use as a mutagen of Schizosaccharomyces pombe.
Article in Methods (San Diego, Calif.), 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Zinc-finger BED domains drive the formation of the active Hermes transpososome by asymmetric DNA binding.Nature communications · 2023Article
- Genome-wide screens in yeast models towards understanding chronological lifespan regulation.Briefings in functional genomics · 2022Article
- Comparing the utility of in vivo transposon mutagenesis approaches in yeast species to infer gene essentiality.Current genetics · 2020Article
- Fitness Landscape of the Fission Yeast Genome.Molecular biology and evolution · 2019Article
- Glycan Alteration Imparts Cellular Resistance to a Membrane-Lytic Anticancer Peptide.Cell chemical biology · 2017Article
- The Paf1 complex factors Leo1 and Paf1 promote local histone turnover to modulate chromatin states in fission yeast.EMBO reports · 2015Article
- An Ancient Yeast for Young Geneticists: A Primer on the Schizosaccharomyces pombe Model System.Genetics · 2015Review
- Serial number tagging reveals a prominent sequence preference of retrotransposon integration.Nucleic acids research · 2014Article
- Article
- A piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe.Nucleic acids research · 2011Article
- DNA transposon Hermes inserts into DNA in nucleosome-free regions in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Transposon mutagenesis allows for the discovery and characterization of genes by creating mutations that can be easily mapped and sequenced. Moreover, this method allows for a relatively unbiased approach to isolating genes of interest. Recently, a system of transposon based mutagenesis for Schizosaccharomyces pombe became available. This mutagenesis relies on Hermes, a DNA transposon from the house fly that readily integrates into the chromosomes of S. pombe. The Hermes system is distinct from the retrotransposons of S. pombe because it efficiently integrates into open reading frames. To mutagenize S. pombe, cells are transformed with a plasmid that contains a drug resistance marker flanked by the terminal inverted repeats of Hermes. The Hermes transposase expressed from a second plasmid excises the resistance marker with the inverted repeats and inserts this DNA into chromosomal sites. After S. pombe with these two plasmids grow 25 generations, approximately 2% of the cells contain insertions. Of the cells with insertions, 68% contain single integration events. The protocols listed here provide the detailed information necessary to mutagenize a strain of interest, screen for specific phenotypes, and sequence the positions of insertion.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.