ArticleMolecular cell2009
Insights into branch nucleophile positioning and activation from an orthogonal pre-mRNA splicing system in yeast.
Article in Molecular cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
30 citing papers in PubMed, 43 citations in OpenAlex.
- The human branchpoint-interacting stem-loop sequence and structure regulates U2 snRNA expression, branchpoint recognition, and the transcriptome.Nucleic acids research · 2026Article
- A common structural mechanism for RNA recognition by the SF3B complex in mRNA splicing and export.Nucleic acids research · 2025Article
- The debranching enzyme Dbr1 regulates lariat turnover and intron splicing.Nature communications · 2024Article
- Circular stable intronic RNAs possess distinct biological features and are deregulated in bladder cancer.NAR cancer · 2023Article
- Structural studies of the spliceosome: Bridging the gaps.Current opinion in structural biology · 2022Review
- Structural basis of branch site recognition by the human spliceosome.Science (New York, N.Y.) · 2022Article
- Evidence for complex dynamics during U2 snRNP selection of the intron branchpoint.Nucleic acids research · 2021Article
- Article
- Genome-wide profiling of prognosis-related alternative splicing signatures in sarcoma.Annals of translational medicine · 2019Article
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Dynamic stacking of an expected branch point adenosine in duplexes containing pseudouridine-modified or unmodified U2 snRNA sites.Biochemical and biophysical research communications · 2019Article
- Functional analysis of Hsh155/SF3b1 interactions with the U2 snRNA/branch site duplex.RNA (New York, N.Y.) · 2018Article
- Unexplored Molecular Features of theFrontiers in cellular and infection microbiology · 2018Article
- A central role of Cwc25 in spliceosome dynamics during the catalytic phase of pre-mRNA splicing.RNA (New York, N.Y.) · 2017Article
- Large-scale analysis of branchpoint usage across species and cell lines.Genome research · 2017Article
- Alternative splicing of U2AF1 reveals a shared repression mechanism for duplicated exons.Nucleic acids research · 2017Article
- Pseudouridines in U2 snRNA stimulate the ATPase activity of Prp5 during spliceosome assembly.The EMBO journal · 2016Article
- Article
- A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.Genes & development · 2015Article
- The DExD/H-box ATPase Prp2p destabilizes and proofreads the catalytic RNA core of the spliceosome.RNA (New York, N.Y.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The duplex formed between the branch site (BS) of a spliceosomal intron and its cognate sequence in U2 snRNA is important for spliceosome assembly and the first catalytic step of splicing. We describe the development of an orthogonal BS-U2 system in S. cerevisiae in which spliceosomes containing a grossly substituted second-copy U2 snRNA mediate the in vivo splicing of a single reporter transcript carrying a cognate substitution. Systematic use of this approach to investigate requirements for branching catalysis reveals considerable flexibility in the sequence of the BS-U2 duplex and its positioning relative to the catalytic center. Branching efficiency depends on the identity of the branch nucleotide, its position within the BS-U2 duplex, and its distance from U2/U6 helix Ia. These results provide insights into substrate selection during spliceosomal branching catalysis; additionally, this system provides a foundation and tool for future mechanistic splicing research.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.