ArticleBiophysical journal2009
Filamin A is essential for active cell stiffening but not passive stiffening under external force.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
65 citing papers in PubMed, 116 citations in OpenAlex.
- Nuclear Mechanics and Nuclear Mechanotransduction in Cancer Cell Migration and Invasion.Biomolecules · 2026Review
- Target cell cortical tension regulates macrophage trogocytosis.Nature cell biology · 2025Article
- Tissue-dependent mechanosensing by cells derived from human tumors.npj biological physics and mechanics · 2025Article
- Multiplexed Single-Cell Rheology Probing Using Surface Acoustic Waves.Small science · 2024Article
- The Role of Mechanotransduction in Contact Inhibition of Locomotion and Proliferation.International journal of molecular sciences · 2024Review
- From stress fiber to focal adhesion: a role of actin crosslinkers in force transmission.Frontiers in cell and developmental biology · 2024Review
- Changes in cell surface excess are coordinated with protrusion dynamics during 3D motility.Biophysical journal · 2023Article
- Nanomechanical Characterization of Ovarian Cancer Cell Lines as a Marker of Response to 2c Treatment.International journal of molecular sciences · 2023Article
- A-to-I RNA editing of Filamin A regulates cellular adhesion, migration and mechanical properties.The FEBS journal · 2022Article
- Nuclear lamin isoforms differentially contribute to LINC complex-dependent nucleocytoskeletal coupling and whole-cell mechanics.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Calcium signaling mediates a biphasic mechanoadaptive response of endothelial cells to cyclic mechanical stretch.Molecular biology of the cell · 2021Article
- Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Article
- The mechanobiome: a goldmine for cancer therapeutics.American journal of physiology. Cell physiology · 2021Review
- The plastic cell: mechanical deformation of cells and tissues.Open biology · 2021Review
- Environmentally controlled magnetic nano-tweezer for living cells and extracellular matrices.Scientific reports · 2020Article
- Qualitative analysis of contribution of intracellular skeletal changes to cellular elasticity.Cellular and molecular life sciences : CMLS · 2020Review
- Stiffness Sensing by Cells.Physiological reviews · 2020Review
- The mechanobiology of actin cytoskeletal proteins during cell-cell fusion.Journal of the Royal Society, Interface · 2019Article
- Tissue cell differentiation and multicellular evolution via cytoskeletal stiffening in mechanically stressed microenvironments.Acta mechanica Sinica = Li xue xue bao · 2019Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
The material properties of a cell determine how mechanical forces are transmitted through and sensed by that cell. Some types of cells stiffen passively under large external forces, but they can also alter their own stiffness in response to the local mechanical environment or biochemical cues. Here we show that the actin-binding protein filamin A is essential for the active stiffening of cells plated on collagen-coated substrates. This appears to be due to a diminished capability to build up large internal contractile stresses in the absence of filamin A. To show this, we compare the material properties and contractility of two human melanoma cell lines that differ in filamin A expression. The filamin A-deficient M2 cells are softer than the filamin A-replete A7 cells, and exert much smaller contractile stresses on the substratum, even though the M2 cells have similar levels of phosphorylated myosin II light chain and only somewhat diminished adhesion strength. In contrast to A7 cells, the stiffness and contractility of M2 cells are insensitive to either myosin-inhibiting drugs or the stiffness of the substratum. Surprisingly, however, filamin A is not required for passive stiffening under large external forces.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.