ArticleBiophysical journal2009
Influence of lamin A on the mechanical properties of amphibian oocyte nuclei measured by atomic force microscopy.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
57 citing papers in PubMed, 110 citations in OpenAlex.
- Nuclear mechanobiology in confined cell migration.Nucleus (Austin, Tex.) · 2026Review
- Prostaglandins regulate the nucleoskeleton during Drosophila border cell migration.bioRxiv : the preprint server for biology · 2026Article
- Nucleus softens during herpesvirus infection.PLoS pathogens · 2026Article
- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Rethinking nuclear shaping: insights from the nuclear drop model.Soft matter · 2024Review
- Indentation induces instantaneous nuclear stiffening and unfolding of nuclear envelope wrinkles.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- 3D photopolymerized microstructured scaffolds influence nuclear deformation, nucleo/cytoskeletal protein organization, and gene regulation in mesenchymal stem cells.APL bioengineering · 2023Article
- Insights in Cell Biomechanics through Atomic Force Microscopy.Materials (Basel, Switzerland) · 2023Review
- Regulation of cellular contractile force, shape and migration of fibroblasts by oncogenes and Histone deacetylase 6.Frontiers in molecular biosciences · 2023Article
- Low lamin A levels enhance confined cell migration and metastatic capacity in breast cancer.Oncogene · 2022Article
- The Nucleus Bypasses Obstacles by Deforming Like a Drop with Surface Tension Mediated by Lamin A/C.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- A Versatile Micromanipulation Apparatus for Biophysical Assays of the Cell Nucleus.Cellular and molecular bioengineering · 2022Article
- The Elephant in the Cell: Nuclear Mechanics and Mechanobiology.Journal of biomechanical engineering · 2022Review
- Nuclear F-actin and Lamin A antagonistically modulate nuclear shape.Journal of cell science · 2022Article
- A Multisensory Network Drives Nuclear Mechanoadaptation.Biomolecules · 2022Review
- Cancer cell mechanobiology: a new frontier for cancer research.Journal of the National Cancer Center · 2022Review
- Viscoelasticity, Like Forces, Plays a Role in Mechanotransduction.Frontiers in cell and developmental biology · 2022Review
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- The Role of Emerin in Cancer Progression and Metastasis.International journal of molecular sciences · 2021Review
- Bend, Push, Stretch: Remarkable Structure and Mechanics of Single Intermediate Filaments and Meshworks.Cells · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nuclear lamina is part of the nuclear envelope (NE). Lamin filaments provide the nucleus with mechanical stability and are involved in many nuclear activities. The functional importance of these proteins is highlighted by mutations in lamin genes, which cause a variety of human diseases (laminopathies). Here we describe a method that allows one to quantify the contribution of lamin A protein to the mechanical properties of the NE. Lamin A is ectopically expressed in Xenopus oocytes, where it is incorporated into the NE of the oocyte nucleus, giving rise to a prominent lamina layer at the inner nuclear membrane. Nuclei are then isolated and probed by atomic force microscopy. From the resulting force curves, stiffness values are calculated and compared with those of control nuclei. Expression of lamin A significantly increases the stiffness of oocyte nuclei in a concentration-dependent manner. Since chromatin adds negligibly to nuclear mechanics in these giant nuclei, this method allows one to measure the contribution of individual NE components to nuclear mechanics.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.