ArticleBiophysical journal2009
Microfluidic investigation reveals distinct roles for actin cytoskeleton and myosin II activity in capillary leukocyte trafficking.
Article in Biophysical journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
34 citing papers in PubMed, 85 citations in OpenAlex.
- Unlocking the therapeutic potential of cellular mechanobiology.Science advances · 2025Review
- Molecular determinants of intrinsic cellular stiffness in health and disease.Biophysical reviews · 2022Review
- Engineering physical microenvironments to study innate immune cell biophysics.APL bioengineering · 2022Review
- Microfluidic investigation of the effect of graphene oxide on mechanical properties of cell and actin cytoskeleton networks: experimental and theoretical approaches.Scientific reports · 2021Article
- Functional Mapping of Adhesiveness on Live Cells Reveals How Guidance Phenotypes Can Emerge From Complex Spatiotemporal Integrin Regulation.Frontiers in bioengineering and biotechnology · 2021Article
- The method to quantify cell elasticity based on the precise measurement of pressure inducing cell deformation in microfluidic channels.MethodsX · 2021Article
- Mechanical adaptation of monocytes in model lung capillary networks.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell Mechanical and Physiological Behavior in the Regime of Rapid Mechanical Compressions that Lead to Cell Volume Change.Small (Weinheim an der Bergstrasse, Germany) · 2020Article
- Stress hormone signaling through β-adrenergic receptors regulates macrophage mechanotype and function.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Understanding flow dynamics, viability and metastatic potency of cervical cancer (HeLa) cells through constricted microchannel.Scientific reports · 2018Article
- Microfluidic generation of transient cell volume exchange for convectively driven intracellular delivery of large macromolecules.Materials today (Kidlington, England) · 2018Article
- Biomimetic post-capillary venule expansions for leukocyte adhesion studies.Scientific reports · 2018Article
- Hydrodynamics in Cell Studies.Chemical reviews · 2018Review
- Flow-Induced Transport of Tumor Cells in a Microfluidic Capillary Network: Role of Friction and Repeated Deformation.Cellular and molecular bioengineering · 2017Article
- Quantitative Deformability Cytometry: Rapid, Calibrated Measurements of Cell Mechanical Properties.Biophysical journal · 2017Article
- Microfluidic Iterative Mechanical Characteristics (iMECH) Analyzer for Single-Cell Metastatic Identification.Analytical methods : advancing methods and applications · 2017Article
- Probing cytoskeletal pre-stress and nuclear mechanics in endothelial cells with spatiotemporally controlled (de-)adhesion kinetics on micropatterned substrates.Cell adhesion & migration · 2017Article
- Cancer cells become less deformable and more invasive with activation of β-adrenergic signaling.Journal of cell science · 2016Article
- Big insights from small volumes: deciphering complex leukocyte behaviors using microfluidics.Journal of leukocyte biology · 2016Review
- On-Chip Quantitative Measurement of Mechanical Stresses During Cell Migration with Emulsion Droplets.Scientific reports · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Circulating leukocyte sequestration in pulmonary capillaries is arguably the initiating event of lung injury in acute respiratory distress syndrome. We present a microfluidic investigation of the roles of actin organization and myosin II activity during the different stages of leukocyte trafficking through narrow capillaries (entry, transit and shape relaxation) using specific drugs (latrunculin A, jasplakinolide, and blebbistatin). The deformation rate during entry reveals that cell stiffness depends strongly on F-actin organization and hardly on myosin II activity, supporting a microfilament role in leukocyte sequestration. In the transit stage, cell friction is influenced by stiffness, demonstrating that the actin network is not completely broken after a forced entry into a capillary. Conversely, membrane unfolding was independent of leukocyte stiffness. The surface area of sequestered leukocytes increased by up to 160% in the absence of myosin II activity, showing the major role of molecular motors in microvilli wrinkling and zipping. Finally, cell shape relaxation was largely independent of both actin organization and myosin II activity, whereas a deformed state was required for normal trafficking through capillary segments.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.