ArticleBioTechniques2009
A novel L1 retrotransposon marker for HeLa cell line identification.
Article in BioTechniques, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
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- Review
- Impact of Training Data, Ground Truth and Shape Variability in the Deep Learning-Based Semantic Segmentation of HeLa Cells Observed with Electron Microscopy.Journal of imaging · 2023Article
- ProInfer: An interpretable protein inference tool leveraging on biological networks.PLoS computational biology · 2023Article
- In Vitro and In Vivo Biological Assays of Dextran Coated Iron Oxide Aqueous Magnetic Fluids.Pharmaceutics · 2023Article
- Preparation and Evaluation ofPharmaceuticals (Basel, Switzerland) · 2023Article
- Content Size-Dependent Alginate Microcapsule Formation Using Centrifugation to Eliminate Empty Microcapsules for On-Chip Imaging Cell Sorter Application.Micromachines · 2022Article
- Review
- Label-free, non-invasive, and repeatable cell viability bioassay using dynamic full-field optical coherence microscopy and supervised machine learning.Biomedical optics express · 2022Article
- Cervical anticancer activities ofVeterinary world · 2022Article
- Phthalocyanine and Its Formulations: A Promising Photosensitizer for Cervical Cancer Phototherapy.Pharmaceutics · 2021Review
- Naproxen release aspect from boron-doped carbon nanodots as a bifunctional agent in cancer therapy.RSC advances · 2021Article
- Preparation and Characterization of Dextran Coated Iron Oxide Nanoparticles Thin Layers.Polymers · 2021Article
- Development of an Acid-Labile Ketal Linked Amphiphilic Block Copolymer Nanoparticles for pH-Triggered Release of Paclitaxel.Polymers · 2021Article
- Article
- Melatonin sensitises shikonin-induced cancer cell death mediated by oxidative stress via inhibition of the SIRT3/SOD2-AKT pathway.Redox biology · 2020Article
- The Significance of Biobanking in the Sustainability of Biomedical Research: A Review.Iranian biomedical journal · 2020Review
- Rational synthesis of three-dimensional core-double shell upconversion nanodendrites with ultrabright luminescence for bioimaging application.Chemical science · 2019Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
The HeLa cell line is the oldest, most widely distributed, permanent human cell line. As a nearly ubiquitous inhabitant of laboratories using tissue culture techniques, its aggressive growth characteristics make it a problematic contaminant that can overgrow less robust cell lines. Consequently, HeLa contamination is common in both the research laboratory and cell line repository contexts, and its detection is hampered by the lack of a rapid, sensitive and robust assay. Here we report the development of a HeLa-specific DNA diagnostic test: a single duplex detection PCR assay targeting an L1 retrotransposon insertion. All HeLa clones from a geographically diverse panel were positive by this assay, and the particular L1 insertion we identified appears to be unique to the HeLa cell line. The assay can detect very low levels of HeLa contamination (<1%), and can be performed on un-purified cell pellets, allowing rapid routine screening.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.