ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2009
Single nucleotide polymorphism genotyping in polyploid wheat with the Illumina GoldenGate assay.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 132 papers.
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Who cites it
132 citing papers in PubMed.
- MKDESIGNER and TASEQ: a set of tools for plant genotyping by targeted amplicon sequencing.BMC bioinformatics · 2025Article
- Genome-wide association study identifies QTL and candidate genes for grain size and weight in a Triticum turgidum collection.The plant genome · 2025Article
- Identification of the genetic background of laboratory rats through amplicon-based next-generation sequencing for single-nucleotide polymorphism genotyping.BMC genomic data · 2024Article
- Wheat Water-Soluble Carbohydrate Remobilisation under Water Deficit byCurrent issues in molecular biology · 2023Article
- Evaluation of CRISPR/Cas9 Constructs in Wheat Cell Suspension Cultures.International journal of molecular sciences · 2023Article
- Comparative genomic analysis of 5MFrontiers in plant science · 2023Article
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- Population structure and genome-wide association studies in bread wheat for phosphorus efficiency traits using 35 K Wheat Breeder's Affymetrix array.Scientific reports · 2021Article
- Candidate Genes and Quantitative Trait Loci for Grain Yield and Seed Size in Durum Wheat.Plants (Basel, Switzerland) · 2021Article
- Comparative Analysis Delineates the Transcriptional Resistance Mechanisms for Pod Borer Resistance in the Pigeonpea Wild RelativeInternational journal of molecular sciences · 2020Article
- Genetic architecture of agronomic and quality traits in a nested association mapping population of spring wheat.The plant genome · 2020Article
- Development, identification, and characterization of blue-grained wheat-Triticum boeoticum substitution lines.Journal of applied genetics · 2020Article
- Genome-wide introgression from a bread wheat × Lophopyrum elongatum amphiploid into wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Comparative analysis of chromosome 2A molecular organization in diploid and hexaploid wheat.Molecular biology reports · 2020Article
- SNP markers for early identification of high molecular weight glutenin subunits (HMW-GSs) in bread wheat.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Article
- Rapid identification of homozygosity and site of wild relative introgressions in wheat through chromosome-specific KASP genotyping assays.Plant biotechnology journal · 2020Article
- Recombination between homoeologous chromosomes induced in durum wheat by the Aegilops speltoides Su1-Ph1 suppressor.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Breeding for dual-purpose wheat varieties using marker-trait associations for biomass yield and quality traits.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2019Article
- Construction of a genetic linkage map ofBreeding science · 2019Article
72 more citing papers are in PubMed but not listed here.
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3 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Single nucleotide polymorphisms (SNPs) are indispensable in such applications as association mapping and construction of high-density genetic maps. These applications usually require genotyping of thousands of SNPs in a large number of individuals. Although a number of SNP genotyping assays are available, most of them are designed for SNP genotyping in diploid individuals. Here, we demonstrate that the Illumina GoldenGate assay could be used for SNP genotyping of homozygous tetraploid and hexaploid wheat lines. Genotyping reactions could be carried out directly on genomic DNA without the necessity of preliminary PCR amplification. A total of 53 tetraploid and 38 hexaploid homozygous wheat lines were genotyped at 96 SNP loci. The genotyping error rate estimated after removal of low-quality data was 0 and 1% for tetraploid and hexaploid wheat, respectively. Developed SNP genotyping assays were shown to be useful for genotyping wheat cultivars. This study demonstrated that the GoldenGate assay is a very efficient tool for high-throughput genotyping of polyploid wheat, opening new possibilities for the analysis of genetic variation in wheat and dissection of genetic basis of complex traits using association mapping approach.
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