Evidence map›Paper›PMID 19448747›Full record

ArticleBiochemistry and cell biology = Biochimie et biologie cellulaire2009

Quantitative determination of urea concentrations in cell culture medium.

Robert J X Zawada, Peggy Kwan, Kellen L Olszewski, Manuel Llinas, Shu-Gui Huang

Open access · greenAbstract read
In one paragraph

Article in Biochemistry and cell biology = Biochimie et biologie cellulaire, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
0.5field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 65 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Robert J X ZawadaBioAssay Systems LLC, Hayward, CA, USA.
Peggy Kwan
Kellen L Olszewski
Manuel Llinas
Shu-Gui Huang
Princeton University · US

Funding

Novel antimalarial strategies using metabolomic network discoveryDP2OD001315 · OD · PRINCETON UNIVERSITY · PI LLINAS, MANUEL · 2007 to 2007
$2.4M
NIH HHS DP2 OD001315
6 · The paper itself

Abstract

Urea is the major nitrogenous end product of protein metabolism in mammals. Here, we describe a quantitative, sensitive method for urea determination using a modified Jung reagent. This assay is specific for urea and is unaffected by ammonia, a common interferent in tissue and cell cultures. We demonstrate that this convenient colorimetric microplate-based, room temperature assay can be applied to determine urea synthesis in cell culture.

Indexed as

AnimalsCalibrationCulture MediaPlasmodium falciparumReference StandardsUreaCulture MediaUrea

Identifiers

PMID19448747
PMCPMC2804236
OpenAlexW2118329387

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.