ArticleNature2009
dUTP incorporation into genomic DNA is linked to transcription in yeast.
Article in Nature, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- ERCC2 mutations alter the genomic distribution pattern of somatic mutations and are independently prognostic in bladder cancer.Cell genomics · 2024Article
- Dynamic alternative DNA structures in biology and disease.Nature reviews. Genetics · 2023Review
- Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method.Nature communications · 2022Article
- Efficient DNA fluorescence labeling via base excision trapping.Nature communications · 2022Article
- Giardia intestinalis thymidine kinase is a high-affinity enzyme crucial for DNA synthesis and an exploitable target for drug discovery.The Journal of biological chemistry · 2022Article
- The activity of yeast Apn2 AP endonuclease at uracil-derived AP sites is dependent on the major carbon source.Current genetics · 2021Article
- Genome-wide alterations of uracil distribution patterns in human DNA upon chemotherapeutic treatments.eLife · 2020Article
- New insights into abasic site repair and tolerance.DNA repair · 2020Review
- The etiology of uracil residues in the Saccharomyces cerevisiae genomic DNA.Current genetics · 2019Review
- Unscheduled DNA synthesis leads to elevated uracil residues at highly transcribed genomic loci in Saccharomyces cerevisiae.PLoS genetics · 2018Article
- Ribonucleotides and Transcription-Associated Mutagenesis in Yeast.Journal of molecular biology · 2017Review
- Def1 and Dst1 play distinct roles in repair of AP lesions in highly transcribed genomic regions.DNA repair · 2017Article
- Review
- Synonymous Genetic Variation in Natural Isolates of Escherichia coli Does Not Predict Where Synonymous Substitutions Occur in a Long-Term Experiment.Molecular biology and evolution · 2015Article
- Genetic instability in budding and fission yeast-sources and mechanisms.FEMS microbiology reviews · 2015Review
- An underlying mechanism for the increased mutagenesis of lagging-strand genes in Bacillus subtilis.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Rad9 interacts with Aft1 to facilitate genome surveillance in fragile genomic sites under non-DNA damage-inducing conditions in S. cerevisiae.Nucleic acids research · 2014Article
- Genomic uracil homeostasis during normal B cell maturation and loss of this balance during B cell cancer development.Molecular and cellular biology · 2014Article
- High resolution mapping of modified DNA nucleobases using excision repair enzymes.Genome research · 2014Article
- Sources of DNA double-strand breaks and models of recombinational DNA repair.Cold Spring Harbor perspectives in biology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Highly activated transcription is associated with eukaryotic genome instability, resulting in increased rates of mitotic recombination and mutagenesis. The association between high transcription and genome stability is probably due to a variety of factors including an enhanced accumulation of DNA damage, transcription-associated supercoiling, collision between replication forks and the transcription machinery, and the persistence of RNA-DNA hybrids. In the case of transcription-associated mutagenesis, we previously showed that there is a direct proportionality between the level of transcription and the mutation rate in the yeast Saccharomyces cerevisiae, and that the molecular nature of the mutations is affected by highly activated transcription. Here we show that the accumulation of apurinic/apyrimidinic sites is greatly enhanced in highly transcribed yeast DNA. We further demonstrate that most apurinic/apyrimidinic sites in highly transcribed DNA are derived from the removal of uracil, the presence of which is linked to direct incorporation of dUTP in place of dTTP. These results show an unexpected relationship between transcription and the fidelity of DNA synthesis, and raise intriguing cell biological issues with regard to nucleotide pool compartmentalization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.