ArticlePlant physiology2009
Loss of the transit peptide and an increase in gene expression of an ancestral chloroplastic carbonic anhydrase were instrumental in the evolution of the cytosolic C4 carbonic anhydrase in Flaveria.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 54 citations in OpenAlex.
- Transposable elements and the evolutionary origins of C4 gene expression: The story of carbonic anhydrases in Flaveria.Plant physiology · 2026Article
- Parsing the stepwise molecular evolution of C4-associated β-carbonic anhydrase in the genus Flaveria.Plant physiology · 2026Article
- Conserved spatial patterning of gene expression in independent lineages of CThe New phytologist · 2026Article
- Leaf transcriptomes from C3, C3-C4 intermediate, and C4Neurachne species give insights into C4 photosynthesis evolution.Plant physiology · 2024Article
- Spatial expression patterns of genes encoding sugar sensors in leaves of C4 and C3 grasses.Annals of botany · 2023Article
- A Machine Learning Framework Identifies Plastid-Encoded Proteins Harboring C3 and C4 Distinguishing Sequence Information.Genome biology and evolution · 2023Article
- Genome-wide identification, evolution, and expression analysis of carbonic anhydrases genes in soybean (Glycine max).Functional & integrative genomics · 2023Review
- Whole-Genome Duplication Facilitated the Evolution of C4 Photosynthesis in Gynandropsis gynandra.Molecular biology and evolution · 2021Article
- A low CO2-responsive mutant of Setaria viridis reveals that reduced carbonic anhydrase limits C4 photosynthesis.Journal of experimental botany · 2021Article
- Lateral Gene Transfer Acts As an Evolutionary Shortcut to Efficient C4 Biochemistry.Molecular biology and evolution · 2020Article
- Rapid and pervasive development- and tissue-specific homeolog expression partitioning in newly formed inter-subspecific rice segmental allotetraploids.BMC genomics · 2018Article
- Loss of the Chloroplast Transit Peptide from an Ancestral CPlant physiology · 2017Article
- Plant Carbonic Anhydrases: Structures, Locations, Evolution, and Physiological Roles.Molecular plant · 2017Review
- A MEM1-like motif directs mesophyll cell-specific expression of the gene encoding the C4 carbonic anhydrase in Flaveria.Journal of experimental botany · 2017Article
- Growth and development of Arabidopsis thaliana under single-wavelength red and blue laser light.Scientific reports · 2016Article
- An Untranslated cis-Element Regulates the Accumulation of Multiple C4 Enzymes in Gynandropsis gynandra Mesophyll Cells.The Plant cell · 2016Article
- Getting the most out of natural variation in C4 photosynthesis.Photosynthesis research · 2014Review
- Article
- Evolution of the C4 photosynthetic pathway: events at the cellular and molecular levels.Photosynthesis research · 2013Review
- Genome-wide transcriptomic analysis of the sporophyte of the moss Physcomitrella patens.Journal of experimental botany · 2013Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
C(4) photosynthesis has evolved multiple times from ancestral C(3) species. Carbonic anhydrase (CA) catalyzes the reversible hydration of CO(2) and is involved in both C(3) and C(4) photosynthesis; however, its roles and the intercellular and intracellular locations of the majority of its activity differ between C(3) and C(4) plants. To understand the molecular changes underlying the evolution of the C(4) pathway, three cDNAs encoding distinct beta-CAs (CA1, CA2, and CA3) were isolated from the leaves of the C(3) plant Flaveria pringlei. The phylogenetic relationship of the F. pringlei proteins with other embryophyte beta-CAs was reconstructed. Gene expression and protein localization patterns showed that CA1 and CA3 demonstrate high expression in leaves and their products localize to the chloroplast, while CA2 expression is low in all organs examined and encodes a cytosolic enzyme. The roles of the F. pringlei enzymes were considered in light of these results, other angiosperm beta-CAs, and Arabidopsis (Arabidopsis thaliana) "omics" data. All three F. pringlei CAs have orthologs in the closely related C(4) plant Flaveria bidentis, and comparisons of ortholog sequences, expression patterns, and intracellular locations of their products indicated that CA1 and CA2 have maintained their ancestral role in C(4) plants, whereas modifications to the C(3) CA3 gene led to the evolution of the CA isoform that catalyzes the first step in the C(4) photosynthetic pathway. These changes included the loss of the chloroplast transit peptide and an increase in gene expression, which resulted in the high levels of CA activity seen in the cytosol of C(4) mesophyll cells.
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