ArticleApplied and environmental microbiology2009
Genetic modification of carbon catabolite repression in Trichoderma reesei for improved protein production.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers.
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Who cites it
72 citing papers in PubMed, 205 citations in OpenAlex.
- Design and Heterologous Production of Bagworm Silk-Inspired Proteins inComputational and structural biotechnology journal · 2026Article
- Robust Production of Cellulases to Develop a Low-Cost Sugar Platform.Advances in biochemical engineering/biotechnology · 2026Review
- The Transcription Factor PoCon7 Is Essential for Fungal Viability and Regulates Chitinase Gene Expression inInternational journal of molecular sciences · 2025Article
- Novel transcription factor regulating cellulase expression and growth improvement in cellulolytic fungi.Microbial cell factories · 2025Article
- One-Pot Depolymerization of Mixed Plastics Using a Dual Enzyme System.ChemSusChem · 2025Article
- A novel luciferase-based reporter tool to monitor the dynamics of carbon catabolite repression in filamentous fungi.Microbial biotechnology · 2024Article
- Transcriptomic and metabolic changes in Trichoderma reesei caused by mutation in xylanase regulator 1 (xyr1).Biotechnology for biofuels and bioproducts · 2024Article
- Novel Cellulase Screening and Optimal Production from the Wood Decaying Xylariaceae: Daldinia Species.Applied biochemistry and biotechnology · 2024Article
- Proteome profiling of enriched membrane-associated proteins unraveled a novel sophorose and cello-oligosaccharide transporter in Trichoderma reesei.Microbial cell factories · 2024Article
- Promoters and Synthetic Promoters in Trichoderma reesei.Methods in molecular biology (Clifton, N.J.) · 2024Article
- CRISPR/Cas9 mediated gene editing of transcription factor ACE1 for enhanced cellulase production in thermophilic fungus Rasamsonia emersonii.Fungal biology and biotechnology · 2023Article
- Roles of PKAc1 and CRE1 in cellulose degradation, conidiation, and yellow pigment synthesis in Trichoderma reesei QM6a.Biotechnology letters · 2022Article
- Development of an Efficient C-to-T Base-Editing System and Its Application to Cellulase Transcription Factor Precise Engineering in Thermophilic FungusMicrobiology spectrum · 2022Article
- Factors regulating cellulolytic gene expression in filamentous fungi: an overview.Microbial cell factories · 2022Review
- A Combined Study on Optimization,Biochemistry research international · 2022Review
- Carbon catabolite repression involves physical interaction of the transcription factor CRE1/CreA and the Tup1-Cyc8 complex in Penicillium oxalicum and Trichoderma reesei.Biotechnology for biofuels · 2021Article
- Article
- Disruption of alpha-tubulin releases carbon catabolite repression and enhances enzyme production in Trichoderma reesei even in the presence of glucose.Biotechnology for biofuels · 2021Article
- Interdependent recruitment of CYC8/TUP1 and the transcriptional activator XYR1 at target promoters is required for induced cellulase gene expression in Trichoderma reesei.PLoS genetics · 2021Article
- Induction and Repression of Hydrolase Genes inFrontiers in microbiology · 2021Review
12 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellulase and hemicellulase genes of the filamentous fungus Trichoderma reesei have been shown to be under carbon catabolite repression mediated by the regulatory gene cre1. In this study, strains were constructed in which the cre1 gene was either completely removed or replaced by a truncated mutant variant, cre1-1, found previously in the Rut-C30 mutant strain with enhanced enzyme production capability. The T. reesei transformants with either deletion or truncation of cre1 had clearly altered colony morphology compared with the parental strains, forming smaller colonies and fewer aerial hyphae and spores. Liquid cultures in a medium with glucose as a carbon source showed that the transformants were derepressed in cellulase and hemicellulase production. Interestingly, they also produced significantly elevated levels of these hydrolytic enzymes in fermentations carried out in a medium inducing the hydrolase genes. This suggests that cre1 acts as a modulator of cellulase and hemicellulase gene expression under both noninducing and inducing conditions. There was no phenotypic difference between the Deltacre1 and cre1-1 mutant strains in any of the experiments done, indicating that the cre1-1 gene is practically a null allele. The results of this work indicate that cre1 is a valid target gene in strain engineering for improved enzyme production in T. reesei.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.