ArticleApplied and environmental microbiology2009
Substrate specificity of a mannose-6-phosphate isomerase from Bacillus subtilis and its application in the production of L-ribose.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 54 citations in OpenAlex.
- Article
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- Phosphate sugar isomerases and their potential for rare sugar bioconversion.Journal of microbiology (Seoul, Korea) · 2020Review
- Recent advances in properties, production, and applications of L-ribulose.Applied microbiology and biotechnology · 2020Review
- Microbial and enzymatic strategies for the production of L-ribose.Applied microbiology and biotechnology · 2020Review
- Metabolism Shapes the Cell.Journal of bacteriology · 2017Review
- Two-step enzymatic synthesis of 6-deoxy-L-psicose.Tetrahedron letters · 2016Article
- The Extracellular Wall-Bound β-N-Acetylglucosaminidase from Lactobacillus casei Is Involved in the Metabolism of the Human Milk Oligosaccharide Lacto-N-Triose.Applied and environmental microbiology · 2016Article
- Article
- Development of novel sugar isomerases by optimization of active sites in phosphosugar isomerases for monosaccharides.Applied and environmental microbiology · 2013Article
- Crystal structure and substrate specificity of D-galactose-6-phosphate isomerase complexed with substrates.PloS one · 2013Article
- Production of L-ribose from L-ribulose by a triple-site variant of mannose-6-phosphate isomerase from Geobacillus thermodenitrificans.Applied and environmental microbiology · 2012Article
- Structure-based function discovery of an enzyme for the hydrolysis of phosphorylated sugar lactones.Biochemistry · 2012Article
- Overexpression, crystallization and preliminary X-ray diffraction analysis of L-ribose isomerase from Acinetobacter sp. strain DL-28.Acta crystallographica. Section F, Structural biology and crystallization communications · 2011Article
- Substrate specificity of the Bacillus licheniformis lyxose isomerase YdaE and its application in in vitro catalysis for bioproduction of lyxose and glucose by two-step isomerization.Applied and environmental microbiology · 2011Article
- Characterization of a mannose-6-phosphate isomerase from Thermus thermophilus and increased L-ribose production by its R142N mutant.Applied and environmental microbiology · 2011Article
- L-ribose production from L-arabinose by using purified L-arabinose isomerase and mannose-6-phosphate isomerase from Geobacillus thermodenitrificans.Applied and environmental microbiology · 2009Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The uncharacterized gene previously proposed as a mannose-6-phosphate isomerase from Bacillus subtilis was cloned and expressed in Escherichia coli. The maximal activity of the recombinant enzyme was observed at pH 7.5 and 40 degrees C in the presence of 0.5 mM Co(2+). The isomerization activity was specific for aldose substrates possessing hydroxyl groups oriented in the same direction at the C-2 and C-3 positions, such as the d and l forms of ribose, lyxose, talose, mannose, and allose. The enzyme exhibited the highest activity for l-ribulose among all pentoses and hexoses. Thus, L-ribose, as a potential starting material for many L-nucleoside-based pharmaceutical compounds, was produced at 213 g/liter from 300-g/liter L-ribulose by mannose-6-phosphate isomerase at 40 degrees C for 3 h, with a conversion yield of 71% and a volumetric productivity of 71 g liter(-1) h(-1).
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